Molecular Epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex Isolated From Children at the Hospital Infantil de México Federico Gómez
Por:
Mancilla-Rojano, Jetsi, Ochoa, Sara A., Reyes-Grajeda J.P., Flores, Victor, Medina-Contreras, Oscar, Espinosa-Mazariego, Karina, Parra-Ortega, Israel, Rosa-Zamboni D.D.L., Castellanos-Cruz M.D.C., Arellano-Galindo, Jose, Cevallos, Miguel A., Hernandez-Castro, Rigoberto, Xicohtencatl-Cortes, Juan, Cruz-Cordova, Ariadnna
Publicada:
1 ene 2020
Resumen:
The Acinetobacter calcoaceticus-baumannii (Acb) complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. The aim of this study was to determine the frequency of isolation of the species that constitute the Acb complex, as well as their susceptibility to antibiotics, and their distribution at the Hospital Infantil de Mexico Federico Gomez (HIMFG). A total of 88 strains previously identified by Vitek 2®, 40 as Acinetobacter baumannii and 48 as Acb complex were isolated from 52 children from 07, January 2015 to 28, September 2017. A. baumannii accounted for 89.77% (79/88) of the strains; Acinetobacter pittii, 6.82% (6/88); and Acinetobacter nosocomialis, 3.40% (3/88). Most strains were recovered mainly from patients in the intensive care unit (ICU) and emergency wards. Blood cultures (BC) provided 44.32% (39/88) of strains. The 13.63% (12/88) of strains were associated with primary bacteremia, 3.4% (3/88) with secondary bacteremia, and 2.3% (2/88) with pneumonia. In addition, 44.32% (39/88) were multidrug-resistant (MDR) strains and, 11.36% (10/88) were extensively drug-resistant (XDR). All strains amplified the blaOXA-51 gene; 51.13% (45/88), the blaOXA-23 gene; 4.54% (4/88), the blaOXA-24 gene; and 2.27% (2/88), the blaOXA-58 gene. Plasmid profiles showed that the strains had 1–6 plasmids. The strains were distributed in 52 pulsotypes, and 24 showed identical restriction patterns, with a correlation coefficient of 1.0. Notably, some strains with the same pulsotype were isolated from different patients, wards, or years, suggesting the persistence of more than one clone. Twenty-seven sequence types (STs) were determined for the strains based on a Pasteur multilocus sequence typing (MLST) scheme using massive sequencing; the most prevalent was ST 156 (27.27%, 24/88). The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas I-Fb system provided amplification in A. baumannii and A. pittii strains (22.73%, 20/88). This study identified an increased number of MDR strains and the relationship among strains through molecular typing. The data suggest that more than one strain could be causing an infection in some patient. The implementation of molecular epidemiology allowed the characterization of a set of strains and identification of different attributes associated with its distribution in a specific environment. © Copyright © 2020 Mancilla-Rojano, Ochoa, Reyes-Grajeda, Flores, Medina-Contreras, Espinosa-Mazariego, Parra-Ortega, Rosa-Zamboni, Castellanos-Cruz, Arellano-Galindo, Cevallos, Hernández-Castro, Xicohtencatl-Cortes and Cruz-Córdova.
Filiaciones:
Mancilla-Rojano, Jetsi:
Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Facultad de Medicina, Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Subdirecc Gest Invest, Cdmx, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Ciencias Biol, Cdmx, Mexico
Ochoa, Sara A.:
Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Subdirecc Gest Invest, Cdmx, Mexico
Reyes-Grajeda J.P.:
Subdirección de Desarrollo de Aplicaciones Clínicas, Instituto Nacional de Medicina Genómica, Ciudad de México, Mexico
Flores, Victor:
Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Irapuato, Mexico
Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Irapuato, Mexico
Medina-Contreras, Oscar:
Unidad de Investigación Epidemiológica en Endocrinología y Nutrición, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Unided Invest Epidemiol Endocrinol & Nutr, Cdmx, Mexico
Espinosa-Mazariego, Karina:
Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Subdirecc Gest Invest, Cdmx, Mexico
Parra-Ortega, Israel:
Departamento de Laboratorio Clínico, Laboratorio Central, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Dept Lab Clin, Lab Cent, Cdmx, Mexico
Rosa-Zamboni D.D.L.:
Departamento de Epidemiología Hospitalaria, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Castellanos-Cruz M.D.C.:
Departamento de Laboratorio Clínico, Laboratorio Central, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Arellano-Galindo, Jose:
Unidad de investigación en Enfermedades Infecciosas, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Unidad Invest Enfermedades Infecciosas, Subdirecc Gest Invest, Cdmx, Mexico
Cevallos, Miguel A.:
Centro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca, Morelos, Mexico
Hernandez-Castro, Rigoberto:
Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, CDMX, Mexico
Hosp Gen Dr Manuel Gea Gonzalez, Dept Ecol Agentes Patogenos, Cdmx, Mexico
Xicohtencatl-Cortes, Juan:
Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Subdirecc Gest Invest, Cdmx, Mexico
Cruz-Cordova, Ariadnna:
Laboratorio de Investigación en Bacteriología Intestinal, Subdirección de Gestión de la Investigación, Hospital Infantil de México Federico Gómez, CDMX, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Bacteriol Intestinal, Subdirecc Gest Invest, Cdmx, Mexico
Inst Nacl Med Genom, Subdirecc Desarrollo Aplicac Clin, Ciudad De Mexico, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Dept Epidemiol Hosp, Cdmx, Mexico
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