Anthelmintic Activity of Extracts and Active Compounds FromDiospyros anisandraonAncylostoma caninum, Haemonchus placeiand Cyathostomins
Por:
Flota-Burgos, Gabriela Janett, Rosado-Aguilar, Jose Alberto, Rodriguez-Vivas, Roger Ivan, Borges-Argaez, Rocio, Martinez-Ortiz-de-Montellano, Cintli, Gamboa-Angulo, Marcela
Publicada:
23 sep 2020
Categoría:
Veterinary (miscellaneous)
Resumen:
The present study aimed to evaluate the anthelmintic activity of leaf
and bark extracts ofDiospyros anisandracollected during different
seasons and their major constituents on eggs ofAncylostoma caninum,
Haemonchus placei, and cyathostomins. Specifically, the eclosion
inhibition of the methanolic extracts of the leaves and bark ofD.
anisandracollected during the dry and rainy seasons (600-37.5 mu g/ml)
were evaluated in addition to the fractions, sub-fractions (300-37.5 mu
g/ml) and active major constituents (150-2.3 mu g/ml). The rainy season
bark extract had the highest percentage of eclosion inhibition (PEI)
against the evaluated nematodes (>= 90% at 75 mu g/ml) along with high
ovicidal activity (90.0 to 93.4% at 75 mu g/ml). The purification of
the rainy season bark extract showed that its biological activity came
from the non-polarn-hexane fraction (>= 93% at 75 mu g/ml). The
bioguided fractionation pointed to sub-fraction 5 as having the highest
anthelmintic activity against the three evaluated genera of nematodes
(PEI >= 93% at 37.5 mu g/ml). Gas chromatography and mass spectrometry
revealed that the major constituent in sub-fraction 5 was plumbagin.
Upon evaluation, plumbagin was confirmed to be responsible for the
anthelmintic activity ofD. anisandra, with a PEI >= 90% at 2.3 mu g/ml
on the three evaluated nematodes. Additionally, the compounds betulin
and lupeol in the bark ofD. anisandrawere evaluated but presented low
anthelmintic activity (PEI <= 5.3% at 2.3 mu g/ml). In conclusion, the
rainy season bark extract ofD. anisandraexerts a high ovicidal activity
against the eggs of the three studied nematodes. Plumbagin is the active
compound responsible for this activity and represents a potential
alternative for the control of different genera of gastrointestinal
nematodes given the current scenario of anthelmintic resistance.
Filiaciones:
Flota-Burgos, Gabriela Janett:
Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Dept Salud Anim, Merida, Mexico
Rosado-Aguilar, Jose Alberto:
Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Dept Salud Anim, Merida, Mexico
Rodriguez-Vivas, Roger Ivan:
Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Dept Salud Anim, Merida, Mexico
Borges-Argaez, Rocio:
Ctr Invest Cient Yucatan AC, Unidad Biotecnol, Merida, Mexico
Martinez-Ortiz-de-Montellano, Cintli:
Univ Nacl Autonoma Mexico, Dept Parasitol, Mexico City, DF, Mexico
Gamboa-Angulo, Marcela:
Ctr Invest Cient Yucatan AC, Unidad Biotecnol, Merida, Mexico
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