Treatment with Hyaluronic Acid and Collagen-Polyvinylpyrrolidone Improves Extracellular Matrix Assembly for Scarring after Tracheal Resection
Por:
Olmos-Zuniga, J. Raul, Baltazares-Lipp, Matilde, Hernandez-Jimenez, Claudia, Jasso-Victoria, Rogelio, Gaxiola-Gaxiola, Miguel, Silva-Martinez, Mariana, Iniguez-Garcia, Marco Antonio, Gonzalez-Gonzalez, A. Ivan, Vazquez-Minero, Juan Carlos, Luna-Flores, Antonia, Solis-Alanis, Norma, Baltazares-Lipp, Mario Enrique
Publicada:
27 ago 2020
Resumen:
Treatment of tracheal stenosis is occasionally performed in combination
with wound healing modulators to manipulate new extracellular matrix
(ECM) formation and prevent fibrosis. Hyaluronic acid (HA) and
collagen-polyvinylpyrrolidone (collagen-PVP) decrease fibrosis in
experimental tracheal healing. However, they have not been used
clinically as their effect on ECM components, which modify tracheal
scarring, has not been described.Objective. To evaluate the effect of
the application of HA, collagen-PVP, a mixture of HA and collagen-PVP
(HA+collagen-PVP), and mitomycin C on the expression of decorin, matrix
metalloproteinase 1 (MMP1), and MMP9, as well as the type of collagen
and deposits formed in the scar after resection and end-to-end
anastomosis (REEA) of the cervical trachea using an experimental
model.Materials and Methods. Thirty dogs underwent REEA of the cervical
trachea and were treated with different wound healing modulators: group
I (n=6), control; group II (n=6), HA; group III (n=6), collagen-PVP;
group IV (n=6), HA+collagen-PVP; and group V (n=6), mitomycin C. The
dogs were evaluated clinically and endoscopically for 4 weeks.
Subsequently, macroscopic and microscopic changes, expression of ECM
proteins, and collagen deposition in tracheal scars were
analysed.Results. Groups II, III, and IV showed reduced endoscopic,
macroscopic, and microscopic inflammation, improved neovascularization,
high decorin expression (p<0.01, analysis of variance (ANOVA)), and
moderate expression of MMP1 (p<0.003, ANOVA) and type I and III collagen
(p<0.05, Kruskal-Wallis). Groups IV and V developed fewer collagen
deposits (p<0.001, ANOVA).Conclusion. Treatment with HA and collagen-PVP
improved post-REEA healing by increasing neovascularization, stimulating
the expression of decorin, and regulating the expression of MMP1, as
well as type I and III collagen and their deposition.
Filiaciones:
Olmos-Zuniga, J. Raul:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Lung Transplantat Res Unit, Mexico City, DF, Mexico
Baltazares-Lipp, Matilde:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Mexico City, DF, Mexico
Hernandez-Jimenez, Claudia:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Mexico City, DF, Mexico
Jasso-Victoria, Rogelio:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Mexico City, DF, Mexico
Gaxiola-Gaxiola, Miguel:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Lab Morphol, Mexico City, DF, Mexico
Silva-Martinez, Mariana:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Lung Transplantat Res Unit, Mexico City, DF, Mexico
Iniguez-Garcia, Marco Antonio:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Subdirect Surg, Mexico City, DF, Mexico
Gonzalez-Gonzalez, A. Ivan:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Otolaryngol Head & Neck Surg, Mexico City, DF, Mexico
Vazquez-Minero, Juan Carlos:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Subdirect Surg, Mexico City, DF, Mexico
Luna-Flores, Antonia:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, Dept Surg Res, Lung Transplantat Res Unit, Mexico City, DF, Mexico
Solis-Alanis, Norma:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Surg, Mexico City, DF, Mexico
Baltazares-Lipp, Mario Enrique:
Inst Nacl Enfermedades Respiratorias Ismael Cosio, External Consultat Serv, Mexico City, DF, Mexico
|