Changes in the GN/GCof the M segment show positive selection and recombination of one aggressive isolate and two mild isolates of tomato spotted wilt virus
Por:
González-Pacheco B.E., Delaye L., Ochoa D., Rojas R., Silva-Rosales L.
Publicada:
1 ene 2020
Resumen:
We isolated and compared three tomato spotted wilt virus (TSWV) isolates from lettuce (TSWV-Let), pepper (TSWV-Pep), and tomato (TSWV-Tom) from central Mexico to determine their ability to infect a set of eighteen differential plant species from seven families. TWSV-Let was an aggressive isolate with the ability to infect up to 52% of the differential plants, including maize, under greenhouse conditions. The nucleotide (nt) sequences of the three isolates are more than 90% similar in the M and S RNA segments. In the M segment of the TSWV-Let isolate, we detected nt changes in their intergenic region (IGR) and, in the Gc gene, a region containing a recombination site, as well as a synapomorphy associated with one of three sites under positive selection with a change in one aa residue (a cysteine-to-valine mutation). We speculate on the association of these features in the Gc gene with host selection, adaptation, aggressiveness, and ability to infect maize plants. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Filiaciones:
González-Pacheco B.E.:
Depto de Ing. Genética, Cinvestav Unidad Irapuato, Km. 9.6 Lib. Nte. Carr. Irapuato-León, Irapuato, Gto. 36821, Mexico
Delaye L.:
Depto de Ing. Genética, Cinvestav Unidad Irapuato, Km. 9.6 Lib. Nte. Carr. Irapuato-León, Irapuato, Gto. 36821, Mexico
Ochoa D.:
Instituto de Fitosanidad, Colegio de Postgraduados, Carretera México-Texcoco, Km 36.5. Montecillos, Mpio. Texcoco, Edo. Mex., D.F. 56230, Mexico
Rojas R.:
Instituto de Fitosanidad, Colegio de Postgraduados, Carretera México-Texcoco, Km 36.5. Montecillos, Mpio. Texcoco, Edo. Mex., D.F. 56230, Mexico
Silva-Rosales L.:
Depto de Ing. Genética, Cinvestav Unidad Irapuato, Km. 9.6 Lib. Nte. Carr. Irapuato-León, Irapuato, Gto. 36821, Mexico
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