Nucleation and growth inhibition of biological minerals by cementum attachment protein-derived peptide (CAP-pi)
Por:
Montoya G., Lopez K., Arenas J., Zamora C., Hoz L., Romo E., Jiménez K., Arzate H.
Publicada:
1 dic 2020
Ahead of Print:
1 ene 2020
Resumen:
Biomineralization is a highly regulated process where proteins/peptides-crystal interactions contribute to the shaping, phasing and aggregation of minerals. We have identified and synthesized a cementum attachment protein-derived peptide (CAP-pi), which corresponds to amino acids 40–53 of the N-terminal CAP domain (MASSDEDGTNGGAS) and its phosphorylated variant (MASpSpDEDGTNGGASp) (CAP-pip). The peptide is composed of polar and negatively charged amino acids, which are disordered, according to in silico analysis. Our results show that CAP-pi inhibits hydroxyapatite (HA) formation and growth. However, it possesses low capacity to inhibit calcium oxalate crystal growth. CAP-pip showed a stronger inhibitory effect on the formation and growth of HA. As well as a high capacity to inhibit calcium oxalate monohydrate growth, mainly due to adsorption on specific growth faces. Small peptides have many advantages over the full-size protein, including low-cost production and modulation characteristics that allow for structural changes. Our findings suggest that CAP-pip-derived peptide could possess therapeutic potential to prevent or treat pathological calcifications such as renal stones and vascular calcification. © 2020 European Peptide Society and John Wiley & Sons, Ltd.
Filiaciones:
Montoya G.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Lopez K.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Arenas J.:
Institute of Physics, National Autonomous University of Mexico, Mexico City, Mexico
Zamora C.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Hoz L.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Romo E.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Jiménez K.:
Faculty of Chemistry, USAII, National Autonomous University of Mexico, Mexico City, Mexico
Arzate H.:
Laboratory of Periodontal Biology, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
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