Molecular effects of silver nanoparticles on monogenean parasites: Lessons from caenorhabditis elegans
Por:
Pimentel-Acosta C.A., Ramírez-Salcedo J., Morales-Serna F.N., Fajer-ávila E.J., Chávez-Sánchez C., Lara H.H., García-Gasca A.
Publicada:
1 ene 2020
Resumen:
The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Pimentel-Acosta C.A.:
Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
Ramírez-Salcedo J.:
Unidad de Microarreglos, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Mexico City, 04510, Mexico
Morales-Serna F.N.:
Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
CONACYT, Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
Fajer-ávila E.J.:
Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
Chávez-Sánchez C.:
Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
Lara H.H.:
Department of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, United States
García-Gasca A.:
Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, Mexico
Gold
|