Cherry extracts attenuate inflammation and oxidative stress triggered by monosodium urate crystals in THP-1 cells
Por:
Vírgen Gen J.J., Guzmán-Gerónimo R.I., Martínez-Flores K., Martínez-Nava G.A., Fernández-Torres J., Zamudio-Cuevas Y.
Ahead of Print:
1 ene 2020
Resumen:
The microwaves-assisted extraction (MAE) for concentration of cherry phytochemicals has seen explored. Polyphenols from cherries, Prunus avium (L.) L., were extracted using a microwave oven at 2,450 MHz, 453 W for a period of 60 s (T60), and was compared versus an unprocessed MAE extract (T0). The extracts were analyzed for total polyphenols, total anthocyanins, and antioxidant capacity. THP-1 cells were stimulated with monosodium urate (MSU) crystals at 150 µg/ml for 24 hr. Cherry extracts were added to cultures concurrently with MSU or 3 hr before MSU addition as pretreatments. Reactive oxygen species (ROS), IL-1ß levels, and MSU crystal phagocytosis were evaluated. T60 extract showed a higher concentration of polyphenols, anthocyanins, and antioxidant activity than T0 extract. ROS were inhibited using the 1:800 and 1:1,600 (v:v) dilutions from both extracts, even used as pretreatments. IL-1ß levels and MSU crystal phagocytosis were reduced. Cherry is a source of polyphenolic compounds with antioxidant and anti-inflammatory activity. Practical applications: The cherries and a cherry extract obtained via MAE has benefits as a possible coadjuvant to conventional gout therapy due to attenuate the inflammation and the oxidative stress triggered by monosodium urate crystals in THP-1 cells, which mimic an acute episode of gout. © 2020 Wiley Periodicals LLC.
Filiaciones:
Vírgen Gen J.J.:
Instituto de Ciencias Básicas, Universidad Veracruzana, Veracruz, Xalapa, Mexico
Guzmán-Gerónimo R.I.:
Instituto de Ciencias Básicas, Universidad Veracruzana, Veracruz, Xalapa, Mexico
Martínez-Flores K.:
Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra-Ibarra (INR-LGII), México City, Mexico
Martínez-Nava G.A.:
Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra-Ibarra (INR-LGII), México City, Mexico
Fernández-Torres J.:
Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra-Ibarra (INR-LGII), México City, Mexico
Zamudio-Cuevas Y.:
Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra-Ibarra (INR-LGII), México City, Mexico
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