The yin/yang of inflammatory status: Blood-brain barrier regulation during sleep
Por:
Hurtado-Alvarado G., Becerril-Villanueva E., Contis-Montes de Oca A., Domínguez-Salazar E., Salinas-Jazmín N., Pérez-Tapia S.M., Pavon L., Velázquez-Moctezuma J., Gómez-González B.
Publicada:
1 ene 2018
Resumen:
Sleep loss induces a low-grade inflammatory status characterized by a subtle but sustained increase of pro-inflammatory mediators, which are key regulators of blood-brain barrier function. To investigate the influence of inflammatory status on blood-brain barrier dysfunction induced by sleep restriction we performed an experiment using two strains of mice with different immunological backgrounds, C57BL/6 mice that have a predominant pro-inflammatory response and BALB/c mice that have a predominant anti-inflammatory response. Mice were sleep-restricted during 10 days using the flowerpot technique during 20 h per day with 4 h of daily sleep opportunity. The systemic inflammatory status, blood-brain barrier permeability, and the hippocampal expression of neuroinflammatory markers were characterized at the 10th day. Serum levels of TNF and IFN-? increased in sleep-restricted C57BL/6 but not in BALB/c mice; no changes in other cytokines were found. Sleep restriction increased blood-brain barrier permeability in C57BL/6 strain but not in BALB/c. The hippocampus of sleep-restricted C57BL/6 mice exhibited an increase in the expression of the neuroinflammatory markers Iba-1, A 2A adenosine receptor, and MMP-9; meanwhile in sleep-restricted BALB/c mice the expression of this markers was lesser than the control group. These data suggest that cytokines may be playing a key role in modulating blood-brain barrier function during sleep restriction, and probably the effects are related to Iba-1, MMP-9 and A 2A adenosine receptor overexpression. © 2017 Elsevier Inc.
Filiaciones:
Hurtado-Alvarado G.:
Area of Neurosciences, Dept. Biology of Reproduction, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico
Becerril-Villanueva E.:
Dept. Psychoimmunology, National Institute of Psychiatry, “Ramón de la Fuente”, Mexico City, Mexico
Contis-Montes de Oca A.:
Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, Mexico
Domínguez-Salazar E.:
Area of Neurosciences, Dept. Biology of Reproduction, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico
Salinas-Jazmín N.:
Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico
Pérez-Tapia S.M.:
Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico
Dept. Immunology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico
Pavon L.:
Dept. Psychoimmunology, National Institute of Psychiatry, “Ramón de la Fuente”, Mexico City, Mexico
Velázquez-Moctezuma J.:
Area of Neurosciences, Dept. Biology of Reproduction, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico
Gómez-González B.:
Area of Neurosciences, Dept. Biology of Reproduction, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico
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