Analysis of CCR3 expression in corneal neovascularization in a murine model and human corneas
Por:
Haber, Alberto, Polania-Baron, Eduardo J., Bolaños-Jimenez R., Navas, Alejandro, Graue Hernandez, Enrique O., Garfias, Yonathan
Publicada:
1 ene 2020
Resumen:
The aim of this study was to examine the expression of the cytokines and chemokines receptor-3 (CCR3) molecule in endothelial cells and vascular structures in a murine model of corneal neovascularization and in samples of neovascularized human corneas. An immunofluorescence assay using the murine model showed a greater proportion and intensity of CCR3 in the epithelium and corneal subepithelial regions in corneas with neovascularization. In the absence of vascularization, no CCR3 was found. Of the 32 studied tissues, eight were vascularized and 24 were avascular. In the human corneas, vascularized corneas showed positive labeling for CD31 in all the analzedtissues, as well as positive labeling for CCR3. Therefore, all vascularized tissues showed positive coexpression of CCR3 and CD31, whereas none of the avascular corneas showed immunolabeling for either of these receptors. These results suggest that CCR3 could be a possible marker for corneal neovascularization with potential to be a therapeutic target. © 2020 Elsevier Ltd
Filiaciones:
Haber, Alberto:
Department of Cornea, External Disease, and Refractive Surgery, Instituto de Offtalmología “Conde de Valenciana”, Mexico City, Mexico
Inst Offtalmol Conde Valenciana, Dept Cornea External Dis & Refract Surg, Mexico City, DF, Mexico
Polania-Baron, Eduardo J.:
Department of Cornea, External Disease, and Refractive Surgery, Instituto de Offtalmología “Conde de Valenciana”, Mexico City, Mexico
Inst Offtalmol Conde Valenciana, Dept Cornea External Dis & Refract Surg, Mexico City, DF, Mexico
Bolaños-Jimenez R.:
Cell and Tissue Biology, Research Unit, Instituto de Oftalmología “Conde de Valenciana IAP”, Chimalpopoca 14, Col. Obrera, Mexico City, 06800, Mexico
Navas, Alejandro:
Department of Cornea, External Disease, and Refractive Surgery, Instituto de Offtalmología “Conde de Valenciana”, Mexico City, Mexico
Inst Offtalmol Conde Valenciana, Dept Cornea External Dis & Refract Surg, Mexico City, DF, Mexico
Graue Hernandez, Enrique O.:
Department of Cornea, External Disease, and Refractive Surgery, Instituto de Offtalmología “Conde de Valenciana”, Mexico City, Mexico
Inst Offtalmol Conde Valenciana, Dept Cornea External Dis & Refract Surg, Mexico City, DF, Mexico
Garfias, Yonathan:
Department of Biochemistry, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Col. Ciudad Universitaria, Mexico City, 04500, Mexico
Cell and Tissue Biology, Research Unit, Instituto de Oftalmología “Conde de Valenciana IAP”, Chimalpopoca 14, Col. Obrera, Mexico City, 06800, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Av Univ 3000,Col Ciudad Univ, Mexico City 04500, DF, Mexico
Inst Oftalmol Conde Valenciana IAP, Res Unit, Cell & Tissue Biol, Chimalpopoca 14, Mexico City 06800, DF, Mexico
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