Toxicity and antimicrobial effect of silver nanoparticles in swine sperms
Por:
Perez-Duran, Francisco, Acosta-Torres, Laura Susana, Serrano-Diaz, Paloma Netzayeli, Toscano-Torres, Irma Arcelia, Olivo-Zepeda, Ingrid Brenda, Garcia-Caxin, Edwin, Nunez-Anita, Rosa Elvira
Publicada:
3 jul 2020
Resumen:
Bacterial contamination in swine semen affects the quality and longevity
of sperm and consequently fertility is reduced. Antibiotics have been
used to prevent bacterial growth, but the frequency of bacterial
resistance to various antibiotics are increasing. Silver nanoparticles
(AgNPs) of 10-20 nm in size have shown a biocide effect in bacteria and
fungi microorganisms without toxicity to certain mammalian cells. The
goal of this study was to analyze both, antimicrobial activity
againstStaphylococcus aureusand toxicity in swine sperms after 10-20 nm
AgNPs treatment.S. aureusproliferation decreased when concentrations
from 0.4 to 10 mM AgNPs were assayed. Also, sperm viability measured by
mitochondrial metabolism after AgNPs treatment up to a concentration of
10 mM, was viable. In addition, viability determined by membrane
integrity of sperms showed that AgNPs treatment up to a concentration of
10 mM was safe. Sperm morphology was evaluated by automated
quantification of proximal and distal drops and whiptails. Data
indicated that AgNPs treatment up to a concentration of 4 mM were
harmless. Finally, sperm capacitation and acrosome reactions were
determined by (chlortetracycline) CTC assay. Data showed that no changes
in sperm capacitation were observed when sperms were treated with 2 mM
of AgNPs, but data showed increased calcium mobilization when treated
with 10 mM AgNPs, which suggested sperm capacitation. Finally, there
were no significant changes encountered on sperm acrosome reaction for
any of the treatments after AgNPs treatment. Taken together, these
results show the potential of AgNPs as an alternative to conventional
antimicrobial agents that are currently used in extenders to preserve
semen required for storage.
Filiaciones:
Perez-Duran, Francisco:
Univ Michoacana, Fac Med Vet & Zootecnia, Tarimbaro, Mexico
Acosta-Torres, Laura Susana:
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super Unidad Leon, Guanajuato, Mexico
Serrano-Diaz, Paloma Netzayeli:
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super Unidad Leon, Guanajuato, Mexico
Toscano-Torres, Irma Arcelia:
Univ Michoacana, Fac Med Vet & Zootecnia, Tarimbaro, Mexico
Olivo-Zepeda, Ingrid Brenda:
Univ Michoacana, Fac Med Vet & Zootecnia, Tarimbaro, Mexico
Garcia-Caxin, Edwin:
Univ Michoacana, Fac Med Vet & Zootecnia, Tarimbaro, Mexico
Nunez-Anita, Rosa Elvira:
Univ Michoacana, Fac Med Vet & Zootecnia, Tarimbaro, Mexico
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