Efficient modification of PAMAM G1 dendrimer surface with beta-cyclodextrin units by CuAAC: impact on the water solubility and cytotoxicity
Por:
Sorroza-Martinez, Kendra, Gonzalez-Mendez, Israel, Martinez-Serrano, Ricardo D., Solano, Jose D., Ruiu, Andrea, Illescas, Javier, Zhu, Xiao Xia, RIVERA, ERNESTO
Publicada:
8 jul 2020
Resumen:
The toxicity of the poly(amidoamine) dendrimers (PAMAM) caused by the
peripheral amino groups has been a limitation for their use as drug
carriers in clinical applications. In this work, we completely modified
the periphery of PAMAM dendrimer generation 1 (PAMAM G1) with
beta-cyclodextrin (beta-CD) units through the Cu(i)-catalyzed
azide-alkyne cycloaddition (CuAAC) to obtain the PAMAM G1-beta-CD
dendrimer with high yield. The PAMAM G1-beta-CD was characterized
by(1)H- and(13)C-NMR and mass spectrometry studies. Moreover, the PAMAM
G1-beta-CD dendrimer showed remarkably higher water solubility than
native beta-CD. Finally, we studied the toxicity of PAMAM G1-beta-CD
dendrimer in four different cell lines, human breast cancer cells (MCF-7
and MDA-MB-231), human cervical adenocarcinoma cancer cells (HeLa) and
pig kidney epithelial cells (LLC-PK1). The PAMAM G1-beta-CD dendrimer
did not present any cytotoxicity in cell lines tested which shows the
potentiality of this new class of dendrimers.
Filiaciones:
Sorroza-Martinez, Kendra:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Gonzalez-Mendez, Israel:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Martinez-Serrano, Ricardo D.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Solano, Jose D.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Ruiu, Andrea:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Illescas, Javier:
Tecnol Nacl Mexico Inst Tecnol Toluca Av, Av Tecnol S-N, Metepec 52149, Mexico
Zhu, Xiao Xia:
Univ Montreal, Dept Chim, CP 6128,Succursale Ctr Ville, Montreal, PQ H3C 3J7, Canada
RIVERA, ERNESTO:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
|