Glaucacetalin E and galphimidin B from Galphimia glauca and their anxiolytic activity
Por:
Rios, Maria Yolanda, Ortega, Alfredo, Dominguez, Blanca, DECIGA, MYRNA, de la Rosa, Vianey
Publicada:
15 sep 2020
Resumen:
Ethnopharmacological relevance: Galphimia glauca is a Mexican medicinal
plant used to treat anxiety, fear, phobia and stress as it possesses
sedative properties which produce a calming effect. Although some
chemical and pharmacological studies have already been carried out on G.
glauca, there are still new chemical entities from this plant whose
anxiolytic activity should be established.
Aim of the study: To validate the use of G. glauca growing in
Cuernavaca, Morelos, as an anti-stress agent, through the purification
and structural identification of its extracts' chemical constituents;
the analysis of the biogenetic relationship of its chemical compounds,
and its biological evaluation to demonstrate its traditional use as
anxiolytic agents.
Materials and methods: The structures of all isolated compounds were
established based on their spectroscopic and spectrometric data. The
structure of compound 2 was corroborated through X-Ray. The anxiolytic
and sedative-like activities were assessed by the open-field, hole-board
and exploration cylinder test.
Results: The nor-triterpenes glaucacetalin E (1) and galphimidin B (2)
were isolated for the first time along with seven other known compounds,
one of them galphimidin (3), from the CHCl3 fraction of the aerial parts
of Galphimia glauca. The biogenesis of the natural nor-triterpenes
isolated from Galphimia glauca is delineated for the first time starting
from the taraxasteryl cation. Oral administration of CHCl3 fraction and
1-3 compounds produced significant attenuation in the anxiety-response
in cylinder activity, decrease in the ambulatory activity and in head
dipping when compared to the vehicle. However, only the extract enhanced
the pentobarbital-induced hypnosis. Diazepam was used as a positive
control.
Conclusion: Our results suggest that G. glauca growing in Cuernavaca,
Morelos, exerts anxiolytic-like activity due to the presence of the
nor-triterpenes 1-3. These results reinforce the potential use of this
species in the treatment of anxiety.
Filiaciones:
Rios, Maria Yolanda:
Univ Autonoma Estado Morelos, Ctr Invest Quim, IICBA, Cuernavaca 62209, Morelos, Mexico
Ortega, Alfredo:
Univ Nacl Autonoma Mexico, Inst Quim, Cuernavaca, Morelos, Mexico
Dominguez, Blanca:
Univ Autonoma Estado Morelos, Ctr Invest Quim, IICBA, Cuernavaca 62209, Morelos, Mexico
DECIGA, MYRNA:
Inst Politecn Nacl, Secc Estudios Posgrad & Invest, Escuela Super Med, Mexico City, DF, Mexico
de la Rosa, Vianey:
Univ Autonoma Estado Morelos, Ctr Invest Quim, IICBA, Cuernavaca 62209, Morelos, Mexico
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