Artemisia cina 30 CH homeopathic treatment against Haemonchus contortus
Por:
Isabel Higuera-Piedrahita, Rosa, Eugenia Lopez-Arellano, Maria, Lopez-Arellano, Raquel, Cuenca-Verde, Cesar, Alfredo Cuellar-Ordaz, Jorge
Publicada:
1 abr 2020
Resumen:
The anthelmintic resistance problem is widely recognized in sheep
production. Therefore, new methods of control against gastrointestinal
nematodes (GIN) need to be integrated. The aim of this work was to
assess the toxicity of A. cina 30 CH as a homeopathic product against
Haemonchus contortus in in vitro and in vivo assays. A. cina 30 CH was
obtained from a commercial laboratory, and confirmation of artemisinin
as a key ingredient was performed with mass spectrophotometry. The A.
cina 30 CH and the artemisinin pure reagent were used for the inhibition
of egg hatching (IEH) and for the inhibition of larval migration of H.
contortus L3 (ILM). In addition, three groups of 10 naturally infected
lambs with GIN were treated with A. cina 30 CH and albendazole, and 10
were used as control. The parasitic infection was monitored at 0, 7, 14
and 28 d postreatment (PT) to determine the number of eggs per gram
(epg) and FAMACHA index. The in vitro data showed 100 % IEH and 64.7 %
ILM by A. cina 30 CH, and nonlethal activity was observed with the
artemisinin pure reagent. The toxicity of A. cina 30 CH against H.
contortus in infected lambs was observed after 7 d of infection.
Administration of the A. cina 30 CH yielded a 69 % reduction in the epg
at 28 d PT, similar to the albendazole (P<0.05). In conclusion, A. cina
30 CH had the ability to IEH and ILM of H. contortus in in vitro assays
and reduced the number of eggs of H. contortus, which is the primary
parasitic nematode in grazing lambs, thereby reducing infection.
Filiaciones:
Isabel Higuera-Piedrahita, Rosa:
Univ Nacl Autonoma Mexico, Programa Maestria & Doctorado Ciencias Prod & Sal, Carr Cuautitlan Teoloyucan Km 2-5, Cuautitlan, Estado De Mexic, Mexico
Eugenia Lopez-Arellano, Maria:
INIFAP, Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, Jiutepec, Morelos, Mexico
Lopez-Arellano, Raquel:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Cuautitlan, Estado De Mexic, Mexico
Cuenca-Verde, Cesar:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Cuautitlan, Estado De Mexic, Mexico
Alfredo Cuellar-Ordaz, Jorge:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Cuautitlan, Estado De Mexic, Mexico
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