Identification of Autophagy-related Proteins in Lungs From Hypersensitivity Pneumonitis Patients
Por:
Cabrera, Sandra, Rodriguez-Bobadilla, Carolina, Vazquez-Morales, Dulce, Gaxiola, Miguel, Maciel, Mariana, Selman, Moises, PARDO, ANNIE
Publicada:
1 jun 2020
Resumen:
Autophagy has been involved in the pathogenesis of various lung
diseases. However, it is not yet known whether autophagy plays a role in
hypersensitivity pneumonitis (HP). HP is an interstitial lung disease
resulting from exposure to a wide variety of antigens that provoke an
exaggerated immune response in susceptible individuals. The aim of this
study was to explore the localization of autophagy key proteins in lungs
from HP patients and controls by immunohistochemistry and analyze their
expression levels by immunoblot. Macrophages and epithelial cells were
strongly positive for the autophagosome biomarker LC3B
(microtubule-associated protein light chain 3 beta) in HP lungs compared
with controls. A similar pattern was found for the autophagy receptor
p62 and the enzyme ATG4B. Unexpectedly, nuclear p62 signal was also
noticed in macrophages from HP lungs. Regarding ATG5 and ATG7
localization, we observed positive staining in neutrophils, vascular
smooth muscle cells, and endothelial cells. Our findings provide for the
first time evidence that proteins from the autophagy machinery are
highly expressed in the lungs of HP patients and describe the specific
cellular and subcellular localization of LC3B, p62, ATG4B, ATG5, and
ATG7 in HP lungs:
Filiaciones:
Cabrera, Sandra:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Cell Biol, Mexico City 04510, DF, Mexico
Rodriguez-Bobadilla, Carolina:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Cell Biol, Mexico City 04510, DF, Mexico
Vazquez-Morales, Dulce:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Cell Biol, Mexico City 04510, DF, Mexico
Gaxiola, Miguel:
Inst Nacl Enfermedades Resp Ismael Cosio Villegas, Mexico City, DF, Mexico
Maciel, Mariana:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Cell Biol, Mexico City 04510, DF, Mexico
Selman, Moises:
Inst Nacl Enfermedades Resp Ismael Cosio Villegas, Mexico City, DF, Mexico
PARDO, ANNIE:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Cell Biol, Mexico City 04510, DF, Mexico
|