Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals
Por:
Arredondo-Hernandez, Rene, Schmulson, Max, Orduna, Patricia, Lopez-Leal, Gamaliel, Zarate, Angel-Mario, Alanis-Funes, Gerardo, David Alcaraz, Luis, Santiago-Cruz, Rubi, Cevallos, Miguel A., Villa, Antonio R., Ponce-de-Leon Rosales, Samuel, Lopez-Vidal, Yolanda, Consorcio Mexicano Estudio Microbi
Publicada:
20 mar 2020
Resumen:
Irritable bowel syndrome (IBS) is the most frequent functional
gastrointestinal disorder, worldwide, with a high prevalence among
Mestizo Latin Americans. Because several inflammatory disorders appear
to affect this population, a further understanding of host genomic
background variants, in conjunction with colonic mucosa dysbiosis, is
necessary to determine IBS physiopathology and the effects of
environmental pressures. Using a simple polygenic model, host single
nucleotide polymorphisms (SNPs) and the taxonomic compositions of
microbiota were compared between IBS patients and healthy subjects. As
proof of concept, five IBS-Rome III patients and five healthy controls
(HCs) were systematically studied. The human and bacterial intestinal
metagenome of each subject was taxonomically annotated and screened for
previously annotated IBS, ulcerative colitis, and Crohn's
disease-associated SNPs or taxon abundance. Dietary data and fecal
markers were collected and associated with the intestinal microbiome.
However, more than 1,000 variants were found, and at least 76 SNPs
differentiated IBS patients from HCs, as did associations with 4 phyla
and 10 bacterial genera. In this study, we found elements supporting a
polygenic background, with frequent variants, among the Mestizo
population, and the colonic mucosal enrichment of Bacteroides,
Alteromonas, Neisseria, Streptococcus, and Microbacterium, may serve as
a hallmark for IBS.
Filiaciones:
Arredondo-Hernandez, Rene:
Univ Nacl Autonoma Mexico, Fac Med, Lab Microbioma, Div Estudios Posgrad, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Mexico City, DF, Mexico
Schmulson, Max:
Univ Nacl Autonoma Mexico, Fac Med, Lab Higado Pancreas & Motilidad HIPAM, Unidad Invest Med Expt, Mexico City, DF, Mexico
Orduna, Patricia:
Univ Nacl Autonoma Mexico, Fac Med, Lab Microbioma, Div Estudios Posgrad, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Mexico City, DF, Mexico
Lopez-Leal, Gamaliel:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City, DF, Mexico
Zarate, Angel-Mario:
Hosp Gen Mexico City, Mexico City, DF, Mexico
Alanis-Funes, Gerardo:
Tecnol Monterrey, Sch Med & Hlth Sci, Monterrey, Mexico
David Alcaraz, Luis:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City, DF, Mexico
Santiago-Cruz, Rubi:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City, DF, Mexico
Cevallos, Miguel A.:
Univ Nacl Autonoma Mexico, Programa Genom Evolut, Ctr Ciencias Genom, Cuernavaca, Morelos, Mexico
Villa, Antonio R.:
Univ Nacl Autonoma Mexico, Fac Med, Lab Microbioma, Div Estudios Posgrad, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Mexico City, DF, Mexico
Ponce-de-Leon Rosales, Samuel:
Univ Nacl Autonoma Mexico, Fac Med, Lab Microbioma, Div Estudios Posgrad, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Mexico City, DF, Mexico
Lopez-Vidal, Yolanda:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City, DF, Mexico
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