Actin depolymerizing factor modulates Rhizobial infection and nodule organogenesis in common bean
Por:
Ortega-Ortega Y., Carrasco-Castilla J., Juárez-Verdayes M.A., Toscano-Morales R., Fonseca-García C., Nava N., Cárdenas L., Quinto C.
Publicada:
1 ene 2020
Resumen:
Actin plays a critical role in the rhizobium–legume symbiosis. Cytoskeletal rearrangements and changes in actin occur in response to Nod factors secreted by rhizobia during symbiotic interactions with legumes. These cytoskeletal rearrangements are mediated by diverse actin-binding proteins, such as actin depolymerization factors (ADFs). We examined the function of an ADF in the Phaseolus vulgaris–rhizobia symbiotic interaction (PvADFE). PvADFE was preferentially expressed in rhizobia-inoculated roots and nodules. PvADFE promoter activity was associated with root hairs harbouring growing infection threads, cortical cell divisions beneath root hairs, and vascular bundles in mature nodules. Silencing of PvADFE using RNA interference increased the number of infection threads in the transgenic roots, resulting in increased nodule number, nitrogen fixation activity, and average nodule diameter. Conversely, overexpression of PvADFE reduced the nodule number, nitrogen fixation activity, average nodule diameter, as well as NODULE INCEPTION (NIN) and EARLY NODULIN2 (ENOD2) transcript accumulation. Hence, changes in ADFE transcript levels affect rhizobial infection and nodulation, suggesting that ADFE is fine-tuning these processes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Ortega-Ortega Y.:
Departamento de Biociencias y Agrobiotecnología, Centro de Investigación en Química Aplicada-CONACYT, Saltillo, Coahuila 25294, Mexico
Ctr Invest Quim Aplicada CONACYT, Dept Biociencias & Agrobiotecnol, Saltillo 25294, Coahuila, Mexico
Carrasco-Castilla J.:
Instituto Politécnico Nacional, Centro de Estudios Científicos y Tecnológicos, 17 León, León, Guanajuato 37358, Mexico
Inst Politecn Nacl, Ctr Estudios Cient & Tecnol 17 Leon, Leon 37358, Guanajuato, Mexico
Juárez-Verdayes M.A.:
Departamento de Docencia, Universidad Autónoma Agraria Antonio Narro, Saltillo, Coahuila 25315, Mexico
Univ Autonoma Agr Antonio Narro, Dept Docencia, Saltillo 25315, Coahuila, Mexico
Toscano-Morales R.:
Department of Plant Biology, College of Biological Sciences, University of California, Davis, Davis, CA 95616, United States
Univ Calif Davis, Coll Biol Sci, Dept Plant Biol, Davis, CA 95616 USA
Fonseca-García C.:
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Cuernavaca, Morelos 62210, Mexico
UNAM, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico
Nava N.:
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Cuernavaca, Morelos 62210, Mexico
UNAM, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico
Cárdenas L.:
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Cuernavaca, Morelos 62210, Mexico
UNAM, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico
Quinto C.:
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Cuernavaca, Morelos 62210, Mexico
UNAM, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico
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