Cementum protein 1-derived peptide (CEMP 1-p1) modulates hydroxyapatite crystal formation in vitro
Por:
Montoya, Gonzalo, Correa, Rodrigo, Arenas, Jesus, Hoz, Lia, Romo, Enrique, Arroyo, Rita, Zeichner-David, Margarita, Arzate, Higinio
Publicada:
1 oct 2019
Ahead of Print:
1 ago 2019
Resumen:
A cementum protein 1-derived peptide (CEMP1-p1) consisting of 20 amino
acids from the CEMP1's N-terminus region: MGTSSTDSQQAGHRRCSTSN, and its
role on the mineralization process in a cell-free system, was
characterized. CEMP1-p1's physicochemical properties, crystal formation,
and hydroxyapatite (HA) nucleation assays were performed. Crystals
induced by CEMP1-p1 were analyzed by scanning electron microscopy,
Fourier-transform infrared spectroscopy-attenuated total reflectance
(FTIR-ATR), X-ray diffraction (XRD), high resolution transmission
electron microscopy (HRTEM), and atomic force microscopy. The results
indicate that CEMP1-p1 lacks secondary structure, forms nanospheres that
organize into three-dimensional structures, possesses affinity to HA,
and induces its nucleation. CEMP1-p1 promotes the formation of spherical
structures composed by densely packed prism-like crystals, which
revealed a Ca/P ratio of 1.56, corresponding to HA. FTIR-ATR showed
predominant spectrum peaks that correspond and are characteristic of HA
and octacalcium phosphate (OCP). Analysis by XRD indicates that the
crystals show planes with a preferential crystalline orientation for HA
and for OCP. HRTEM showed interplanar distances that correspond to
crystalline planes of HA and OCP. Crystals are composed by superimposed
lamellae, which exhibit epitaxial growth, and each layer of the crystals
is structured by nanocrystals. This study reveals that CEMP1-p1
regulates HA crystal formation, somehow mimicking the in vivo process of
mineralized tissues bioformation.
Filiaciones:
Montoya, Gonzalo:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Correa, Rodrigo:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Arenas, Jesus:
Instituto de Fisica, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
Hoz, Lia:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Romo, Enrique:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Arroyo, Rita:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Zeichner-David, Margarita:
Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, United States
Arzate, Higinio:
Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
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