Acetone effects on Buddleja scordioides polyphenol extraction process and assessment of their cellular antioxidant capacity and anti-inflammatory activity
Por:
Villegas-Novoa, Cecilia, Gallegos-Infante, Jose A., Gonzalez-Laredo, Ruben F., Garcia-Carranca, Alejandro M., Herrera-Rocha, Karen M., Jacobo-Karam, Janett S., Moreno-Jimenez, Martha R., Rocha-Guzman, Nuria E.
Publicada:
1 ene 2019
Resumen:
The use of water, acetone, and hydroacetonic solutions as an extraction solvent for polyphenol compounds from Buddleja scordioides has been investigated. The phenolic profiles determined by liquid chromatography—electrospray ionization—mass spectrometry/mass spectrometry (LC–ESI–MS/MS) were compared with those obtained with the traditional aqueous infusion used for the treatment of gastrointestinal disorders. It was evaluated the effects of polyphenol extract at physiologically relevant conditions against H2O2-induced oxidative stress via attenuation of reactive oxygen species (ROS) levels and lipopolysaccharide (LPS)-induced inflammation in human HT-29 cells. The use of hydroacetonic solutions allows obtaining phytochemical extracts enriched with constituents that have antioxidant and anti-inflammatory activity in intestinal cells. This suggests that polyphenols of low and middle polarity from Buddleja scordioides may be better extracted with hydroacetonic solutions, and showing high nutraceutical potential to reduce oxidative stress associated with the onset and progress of inflammatory diseases. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Filiaciones:
Villegas-Novoa, Cecilia:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
Gallegos-Infante, Jose A.:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
Gonzalez-Laredo, Ruben F.:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
Garcia-Carranca, Alejandro M.:
Laboratorio de Virus y Cáncer, Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Cancerología, Secretaría de Salud, Av. 12 San Fernando 22, Col. Sección XVI, Del Tlalpan, CDMX 14080, Mexico
Univ Nacl Autonoma Mexico, Lab Virus & Canc, Unidad Invest Biomed Canc, Inst Invest Biomed,Inst Nacl Cancerol,Secretaria, Av 12 San Fernando 22,Col Secc 16, Cdmx 14080, Mexico
Herrera-Rocha, Karen M.:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
Jacobo-Karam, Janett S.:
Facultad de Medicina y Nutrición, Universidad Juárez del Estado de Durango, Av. Universidad y Fanny Anitúa s/n, Durango, Dgo. 34000, Mexico
Univ Juarez Estado Durango, Fac Med & Nutr, Av Univ & Fanny Anitua S-N, Durango 34000, Dgo, Mexico
Moreno-Jimenez, Martha R.:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
Rocha-Guzman, Nuria E.:
Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., Durango, Dgo. 34080, Mexico
TecNM Inst Tecnol Durango, Dept Ingn Quim & Bioquim, Res Grp Funct Foods & Nutraceut, Felipe Pescador 1830 Ote, Durango 34080, Dgo, Mexico
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