Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
Por:
Vazquez-Muñoz R., Meza-Villezcas A., Fournier P.G.J., Soria-Castro E., Juarez-Moreno K., Gallego-Hernández A.L., Bogdanchikova N., Vazquez-Duhalt R., Huerta-Saquero A.
Publicada:
1 ene 2019
Categoría:
Multidisciplinary
Resumen:
The ability of microorganisms to generate resistance outcompetes with the generation of new and efficient antibiotics; therefore, it is critical to develop novel antibiotic agents and treatments to control bacterial infections. An alternative to this worldwide problem is the use of nanomaterials with antimicrobial properties. Silver nanoparticles (AgNPs) have been extensively studied due to their antimicrobial effect in different organisms. In this work, the synergistic antimicrobial effect of AgNPs and conventional antibiotics was assessed in Gram-positive and Gram-negative bacteria. AgNPs minimal inhibitory concentration was 10–12 µg mL-1 in all bacterial strains tested, regardless of their different susceptibility against antibiotics. Interestingly, a synergistic antimicrobial effect was observed when combining AgNPs and kanamycin according to the fractional inhibitory concentration index, FICI: <0.5), an additive effect by combining AgNPs and chloramphenicol (FICI: 0.5 to 1), whereas no effect was found with AgNPs and ß-lactam antibiotics combinations. Flow cytometry and TEM analysis showed that sublethal concentrations of AgNPs (6–7 µg mL-1) altered the bacterial membrane potential and caused ultrastructural damage, increasing the cell membrane permeability. No chemical interactions between AgNPs and antibiotics were detected. We propose an experimental supported mechanism of action by which combinatorial effect of antimicrobials drives synergy depending on their specific target, facilitated by membrane alterations generated by AgNPs. Our results provide a deeper understanding about the synergistic mechanism of AgNPs and antibiotics, aiming to combat antimicrobial infections efficiently, especially those by multi-drug resistant microorganisms, in order to mitigate the current crisis due to antibiotic resistance. © 2019 Vazquez-Muñoz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Filiaciones:
Vazquez-Muñoz R.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
Ctr Invest Cient & Educ Super Ensenada, Ensenada, Baja California, Mexico
Meza-Villezcas A.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
Ctr Invest Cient & Educ Super Ensenada, Ensenada, Baja California, Mexico
Fournier P.G.J.:
Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico
Soria-Castro E.:
Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, Mexico
Inst Nacl Cardiol Ignacio Chavez, Ciudad De Mexico, Mexico
Juarez-Moreno K.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
Gallego-Hernández A.L.:
Departamento de Investigación en Física, Universidad de Sonora, Hermosillo, Sonora, Mexico
Univ Sonora, Dept Invest Fis, Hermosillo, Sonora, Mexico
Bogdanchikova N.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
Vazquez-Duhalt R.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
Huerta-Saquero A.:
Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico
Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Ensenada, Baja California, Mexico
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