Open source automated western blot processor
Por:
Bravo-Martinez, Jorge
Publicada:
1 oct 2019
Resumen:
Researchers in the biomedical area are always involved in methodologies
comprising several processes that are repetitive and time-consuming;
these researchers can take advantage of this time for other more
important things. For many years, the trend for this type of problem has
been automation. One of the routine methodologies used by researchers in
broad areas of basic investigation is the Western blot technique. This
method allows the detection through specific antibodies and the eventual
quantification of the protein of interest in different biological
lysates transferred onto a suitable membrane. This methodology involves
several repetitive processes; one of them is the washing of blots after
incubations with primary and secondary antibodies. The present device
has been designed to automate this process at a low cost. Researchers
must use several tools to carry out the same task at a much higher
price, and more importantly, in a time-consuming process. Although it is
designed for the Western blot, it can be optimized for other cyclic
tasks. (C) 2019 The Author(s). Published by Elsevier Ltd.
Filiaciones:
Bravo-Martinez, Jorge:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, UNAM, Ciudad de México, Mexico
Gold
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