Loss of arginine vasopressin- and vasoactive intestinal polypeptide-containing neurons and glial cells in the suprachiasmatic nucleus of individuals with type 2 diabetes
Por:
Hogenboom, Rick, Kalsbeek, Martin J., Korpel, Nikita L., de Goede, Paul, Koenen, Marit, Buijs, Ruud M., Romijn, Johannes A., Swaab, Dick F., Kalsbeek, Andries, Yi C.-X.
Publicada:
1 ene 2019
Resumen:
Aims/hypothesis: The central pacemaker of the mammalian biological timing system is located within the suprachiasmatic nucleus (SCN) in the anterior hypothalamus. Together with the peripheral clocks, this central brain clock ensures a timely, up-to-date and proper behaviour for an individual throughout the day–night cycle. A mismatch between the central and peripheral clocks results in a disturbance of daily rhythms in physiology and behaviour. It is known that the number of rhythmically expressed genes is reduced in peripheral tissue of individuals with type 2 diabetes mellitus. However, it is not known whether the central SCN clock is also affected in the pathogenesis of type 2 diabetes. In the current study, we compared the profiles of the SCN neurons and glial cells between type 2 diabetic and control individuals. Methods: We collected post-mortem hypothalamic tissues from 28 type 2 diabetic individuals and 12 non-diabetic control individuals. We performed immunohistochemical analysis for three SCN neuropeptides, arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP) and neurotensin (NT), and for two proteins expressed in glial cells, ionised calcium-binding adapter molecule 1 (IBA1, a marker of microglia) and glial fibrillary acidic protein (GFAP, a marker of astroglial cells). Results: The numbers of AVP immunoreactive (AVP-ir) and VIP-ir neurons and GFAP-ir astroglial cells in the SCN of type 2 diabetic individuals were significantly decreased compared with the numbers in the SCN of the control individuals. In addition, the relative intensity of AVP immunoreactivity was reduced in the individuals with type 2 diabetes. The number of NT-ir neurons and IBA1-ir microglial cells in the SCN was similar in the two groups. Conclusions/interpretation: Our data show that type 2 diabetes differentially affects the numbers of AVP- and VIP-expressing neurons and GFAP-ir astroglial cells in the SCN, each of which could affect the daily rhythmicity of the SCN biological clock machinery. Therefore, for effectively treating type 2 diabetes, lifestyle changes and/or medication to normalise central biological clock functioning might be helpful. © 2019, The Author(s).
Filiaciones:
Hogenboom, Rick:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Kalsbeek, Martin J.:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Korpel, Nikita L.:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
de Goede, Paul:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Koenen, Marit:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Buijs, Ruud M.:
Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Cell Biol & Physiol, Mexico City, DF, Mexico
Romijn, Johannes A.:
Department of Medicine, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Dept Med, Amsterdam, Netherlands
Swaab, Dick F.:
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Kalsbeek, Andries:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
Amsterdam Univ Med Ctr UMC, Dept Endocrinol & Metab, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands
Univ Amsterdam, Amsterdam Univ Med Ctr UMC, Lab Endocrinol, Amsterdam Gastroenterol & Metab, Amsterdam, Netherlands
Netherlands Inst Neurosci, Amsterdam, Netherlands
Yi C.-X.:
Department of Endocrinology and Metabolism, Amsterdam University Medical Centers (UMC), Location AMC, Meibergdreef 9, Amsterdam, AZ 1105, Netherlands
Laboratory of Endocrinology, Amsterdam University Medical Centers (UMC), University of Amsterdam, Amsterdam Gastroenterology and Metabolism, Amsterdam, Netherlands
Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands
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