Histochemical Study of the Emergence of Apoptosis and Altered SYCP3 Protein Distribution During the First Spermatogenic Wave in Wistar Rats
Por:
Escobar, Maria L., Echeverria, Olga M., Valenzuela, Yunuen M., Ortiz, Rosario, Torres-Ramirez, Nayeli, Vazquez-Nin, Gerardo H.
Publicada:
1 nov 2019
Resumen:
Apoptosis is a type of cell death responsible for maintaining tissue
homeostasis that can occur in male gonads. The morphological and
biochemical characteristics of apoptosis include cellular contraction,
caspase activation, and DNA fragmentation. Dynamic processes of cell
renewal and differentiation occur inside the seminiferous tubules, which
are regulated by mitosis and meiosis, respectively. During meiosis,
recombination is caused by assembly of the synaptonemal complex, which
involves the participation of constitutive proteins, such as
synaptonemal complex protein-3 (SYCP3). The present study evaluated
germinal cell death in immature male rats and the distribution of the
SYCP3 protein. Our results indicate that as germinal cells progress to
the second meiotic stage, significant numbers of them are eliminated by
apoptosis. We determined that the SYCP3 protein is not always
incorporated into the structure of the synaptonemal complex but rather
forms a nuclear cumulus near the inner nuclear membrane, causing many of
these cells to undergo apoptosis. We propose that both the excess of the
SYCP3 protein and its accumulation during the first meiotic division
could contribute to the cell death of primary spermatocytes during the
first spermatogenic wave in prepubertal Wistar rats. Anat Rec,
302:2082-2092, 2019. (c) 2019 American Association for Anatomy
Filiaciones:
Escobar, Maria L.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
Echeverria, Olga M.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
Valenzuela, Yunuen M.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
Ortiz, Rosario:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
Torres-Ramirez, Nayeli:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
Vazquez-Nin, Gerardo H.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Microscopia Elect, Av Univ 3000, Mexico City 04510, DF, Mexico
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