Nanoscale organization of rotavirus replication machineries
Por:
Garces Suarez, Yasel, Martinez, Jose L., Torres Hernandez, David, Olinca Hernandez, Haydee, Perez-Delgado, Arianna, Mendez, Mayra, Wood, Christopher D., Manuel Rendon-Mancha, Juan, Silva-Ayala, Daniela, Lopez, Susana, Guerrero, Adan, Arias, Carlos F.
Publicada:
25 jul 2019
Resumen:
Rotavirus genome replication and assembly take place in cytoplasmic
electron dense inclusions termed viroplasms (VPs). Previous conventional
optical microscopy studies observing the intracellular distribution of
rotavirus proteins and their organization in VPs have lacked
molecular-scale spatial resolution, due to inherent spatial resolution
constraints. In this work we employed super-resolution microscopy to
reveal the nanometric-scale organization of VPs formed during rotavirus
infection, and quantitatively describe the structural organization of
seven viral proteins within and around the VPs. The observed viral
components are spatially organized as five concentric layers, in which
NSP5 localizes at the center of the VPs, surrounded by a layer of NSP2
and NSP4 proteins, followed by an intermediate zone comprised of the
VP1, VP2, VP6. In the outermost zone, we observed a ring of VP4 and
finally a layer of VP7. These findings show that rotavirus VPs are
highly organized organelles.
Filiaciones:
Garces Suarez, Yasel:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Martinez, Jose L.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Torres Hernandez, David:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Mexico City, DF, Mexico
Olinca Hernandez, Haydee:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Mexico City, DF, Mexico
Perez-Delgado, Arianna:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Mendez, Mayra:
Univ Autonoma Estado Morelos, Ctr Invest Ciencias, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
Wood, Christopher D.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Mexico City, DF, Mexico
Manuel Rendon-Mancha, Juan:
Univ Autonoma Estado Morelos, Ctr Invest Ciencias, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
Silva-Ayala, Daniela:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Harvard Med Sch, Boston, MA 02115 USA
Lopez, Susana:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Guerrero, Adan:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Mexico City, DF, Mexico
Arias, Carlos F.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City, DF, Mexico
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