Transcriptomic and Proteomic Analysis of the Tentacles and Mucus of Anthopleura dowii Verrill, 1869
Por:
Ramirez-Carreto, Santos, Vera-Estrella, Rosario, Portillo-Bobadilla, Tobias, Licea-Navarro, Alexei, Bernaldez-Sarabia, Johanna, Rudiño-Piñera E., Verleyen, Jerome J., Rodriguez, Estefania, Rodriguez-Almazan, Claudia
Publicada:
1 ene 2019
Categoría:
Drug discovery
Resumen:
Sea anemone venom contains a complex and diverse arsenal of peptides and proteins of pharmacological and biotechnological interest, however, only venom from a few species has been explored from a global perspective to date. In the present study, we identified the polypeptides present in the venom of the sea anemone Anthopleura dowii Verrill, 1869 through a transcriptomic and proteomic analysis of the tentacles and the proteomic profile of the secreted mucus. In our transcriptomic results, we identified 261 polypeptides related to or predicted to be secreted in the venom, including proteases, neurotoxins that could act as either potassium (K+) or sodium (Na+) channels inhibitors, protease inhibitors, phospholipases A2, and other polypeptides. Our proteomic data allowed the identification of 156 polypeptides—48 exclusively identified in the mucus, 20 in the tentacles, and 88 in both protein samples. Only 23 polypeptides identified by tandem mass spectrometry (MS/MS) were related to the venom and 21 exclusively identified in the mucus, most corresponding to neurotoxins and hydrolases. Our data contribute to the knowledge of evolutionary and venomic analyses of cnidarians, particularly of sea anemones. © 2019 by the authors.
Filiaciones:
Ramirez-Carreto, Santos:
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Med Mol & Bioproc, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
Vera-Estrella, Rosario:
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
Portillo-Bobadilla, Tobias:
Unidad de Bioinformática, Bioestadística y Biología Computacional. Red de Apoyo a la Investigación, Coordinación de la Investigación Científica, Universidad Nacional Autónoma de México-Instituto Nacional De Ciencias Médicas y Nutrición Salvador Zubirán, Calle Vasco de Quiroga 15, Tlalpan, Ciudad de México, C.P. 14080, Mexico
Univ Nacl Autonoma Mexico, Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Coordinac Invest Cient, Unidad Bioinformat Bioestadist & Biol Computac,Re, Calle Vasco de Quiroga 15, Ciudad De Mexico 14080, Mexico
Licea-Navarro, Alexei:
Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico
CICESE, Dept Innovac Biomed, Carretera Ensenada Tijuana 3918, Ensenada 22860, Baja California, Mexico
Bernaldez-Sarabia, Johanna:
Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico
CICESE, Dept Innovac Biomed, Carretera Ensenada Tijuana 3918, Ensenada 22860, Baja California, Mexico
Rudiño-Piñera E.:
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico
Verleyen, Jerome J.:
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Unidad Univ Secuenciac Mas & Bioinformat, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
Rodriguez, Estefania:
Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, United States
Amer Museum Nat Hist, Div Invertebrate Zool, Cent Pk West & 79th St, New York, NY 10024 USA
Rodriguez-Almazan, Claudia:
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Med Mol & Bioproc, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
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