Molecular and phenotypic characterization of diarrheagenic Escherichia coli isolated from groundwater in rural areas in southern Brazil
Por:
da Silva, Caroline Rodrigues, Sanches, Matheus Silva, Macedo, Kawana Hiromori, Dambrozio A.M.L., Da Rocha S.P.D., Pelayo J.S., Navarro A.
Publicada:
1 ene 2019
Resumen:
Water-borne diseases like diarrheagenic Escherichia coli (DEC)-induced gastroenteritis are major public health problems in developing countries. In this study, the microbiological quality of water from mines and shallow wells was analyzed for human consumption. Genotypic and phenotypic characterization of DEC strains was performed. A total of 210 water samples was analyzed, of which 153 (72.9%) contained total coliforms and 96 (45.7%) E. coli. Of the E. coli isolates, 27 (28.1%) contained DEC genes. The DEC isolates included 48.1% Shiga toxin-producing E. coli (STEC), 29.6% enteroaggregative E. coli (EAEC), 14.9% enteropathogenic E. coli (EPEC), 3.7% enterotoxigenic E. coli (ETEC), and 3.7% enteroinvasive E. coli (EIEC). All the STECs had cytotoxic effects on Vero cells and 14.8% of the DEC isolates were resistant to at least one of the antibiotics tested. All DEC formed biofilms and 92.6% adhered to HEp-2 cells with a prevalence of aggregative adhesion (74%). We identified 25 different serotypes. One EPEC isolate was serotype O44037:H7, reported for the first time in Brazil. Phylogenetically, 63% of the strains belonged to group B1. The analyzed waters were potential reservoirs for DEC and could act as a source for infection of humans. Preventive measures are needed to avoid such contamination. © 2019 IWA Publishing. All rights reserved.
Filiaciones:
da Silva, Caroline Rodrigues:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Univ Estadual Londrina, Dept Microbiol, Rodovia Celso Garcia Cid,Pr 445 Km 380, BR-86057970 Londrina, PR, Brazil
Sanches, Matheus Silva:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Univ Estadual Londrina, Dept Microbiol, Rodovia Celso Garcia Cid,Pr 445 Km 380, BR-86057970 Londrina, PR, Brazil
Macedo, Kawana Hiromori:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Univ Estadual Londrina, Dept Microbiol, Rodovia Celso Garcia Cid,Pr 445 Km 380, BR-86057970 Londrina, PR, Brazil
Dambrozio A.M.L.:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Da Rocha S.P.D.:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Pelayo J.S.:
Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 Km 380, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil
Navarro A.:
Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Salud Publ, Ciudad Univ, Mexico City, DF, Mexico
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