Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome
Por:
Ambrosio, J. R., Palacios-Arreola, I, M., Rios-Valencia, D. G., Reynoso-Ducoing, O., Nava-Castro, K. E., Ostoa-Saloma, P., Morales-Montor, J.
Publicada:
1 sep 2019
Resumen:
Androgens have been shown to exert a cysticidal effect upon Taenia
crassiceps, an experimental model of cysticercosis. To further inquire
into this matter, the Taenia crassiceps model was used to evaluate the
expression of several proteins after testosterone (T4) and
dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps,
parasite extracts were resolved into approximately 130 proteins
distributed in a molecular weight range of 10-250 kDa and isoelectrical
point range of 3-10. The resultant proteomic pattern was analysed, and
significant changes were observed in response to T4 and DHT. Based on
our experience with electrophoretic patterns and proteomic maps of
cytoskeletal proteins, alteration in the expression of isoforms of
actin, tubulin and paramyosin and of other proteins was assessed.
Considering that androgens may exert their biological activity in
taeniids through the non-specific progesterone receptor membrane
component (PGRMC), we harnessed bioinformatics to propose the identity
of androgen-regulated proteins and establish their hypothetical
physiological role in the parasites. These analyses yield a possible
explanation of how androgens exert their cysticidal effects through
changes in the expression of proteins involved in cytoskeletal
rearrangement, dynamic vesicular traffic and transduction of
intracellular signals.
Filiaciones:
Ambrosio, J. R.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Edificio A,2do Piso,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Rios-Valencia, D. G.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Edificio A,2do Piso,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Reynoso-Ducoing, O.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Edificio A,2do Piso,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Nava-Castro, K. E.:
I , Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Dept Ciencias Ambientales, Lab Genotoxicol & Mutagenesis Ambiental, Ciudad De Mexico 04510, Mexico
Ostoa-Saloma, P.:
I , Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Ciudad De Mexico 04510, Mexico
Morales-Montor, J.:
I , Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Ciudad De Mexico 04510, Mexico
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