Identification of lipopeptides from Bacillus strain Q11 with ability to inhibit the germination of Penicillium expansum, the etiological agent of postharvest blue mold disease
Por:
Luis Rodriguez-Chavez, Jose, Suhan Juarez-Campusano, Yara, Delgado G., Pacheco Aguilar J.R.
Publicada:
1 sep 2019
Resumen:
The genus Bacillus produces secondary metabolites among which stand out
compounds of polypeptide nature for their versatility of applications,
one of them is the activity against fungal phytopathogens of
agricultural importance. In the present work was evaluated the
effectiveness of the crude lipopeptide from Bacillus subtilis Q11
against common soil borne pathogens, additionally in vitro and in vivo
assays were carried out to determine the ability to inhibit the
germination of conidia and reduce the blue mold rot induced by
Penicillium expansum in apple (Malus domestica Borkh) fruit. In
antagonistic test, the lipopeptide fraction of Q11 was able to limit the
mycelial growth of Rhizoctonia solani, Sclerotium rolfsii, Penicillium
expansum, Fusarium stilboides, Colletotrichum gleosporides and Botrytis
cinerea, showing higher inhibition at higher lipopeptide concentrations
(10, 20, and 30 mu g disk(-1)); however, it was ineffective against
Pseudomonas syrigae, Xanthomonas campestris, and Clavibacter
michiganensis. On the other hand, the conidia of P. expansum after being
exposed to a concentration of 800 mg L-1 of lipopeptide for 16 h, showed
swelling and germination reduction greater than 90%. In fruit assay
with apples inoculated with a droplet of conidia suspension (200
conidia) of P. expansum, the treatment with the lipopeptide fraction
reduced rot lesion severity, registering the greatest effects at 80 mu
g, which reduced the size of the lesion by more than 60%. The analysis
by infrared spectroscopy, HPLC and MALDI-TOF established that the crude
lipopeptide is a complex formed primarily by iturin-type compounds with
fatty acid chain length of C14-C17 and small proportion of compounds of
fengycin A and B type that differ in the length of the fatty acid chain.
These compounds could be useful in the development of products for
biological control of postharvest blue mold disease in apple.
Filiaciones:
Luis Rodriguez-Chavez, Jose:
Univ Autonoma Queretaro, Fac Quim, Ctr Univ CU Cerro Campanas, Cerro Campanas S-N, Queretaro 76100, Mexico
CONACYT, Mexico City, DF, Mexico
Suhan Juarez-Campusano, Yara:
Univ Autonoma Queretaro, Fac Quim, Ctr Univ CU Cerro Campanas, Cerro Campanas S-N, Queretaro 76100, Mexico
Delgado G.:
Univ Nacl Autonoma Mexico, Inst Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, 04510, Mexico
Pacheco Aguilar J.R.:
Univ Autonoma Queretaro, Fac Quim, Ctr Univ CU Cerro Campanas, Cerro Campanas S-N, Queretaro 76100, Mexico
Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario (CU), Cerro de las Campanas, Cerro de las Campanas s/n, Querétaro, 76100, Mexico
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