Artemisia absinthium-based silver nanoparticles antifungal evaluation against three Candida species
Por:
del Pilar Rodriguez-Torres, Maria, Susana Acosta-Torres, Laura, Armando Diaz-Torres, Luis, Hernandez Padron, Genoveva, Garcia-Contreras, Rene, Millan-Chiu, Blanca E.
Publicada:
1 ago 2019
Resumen:
Silver nanoparticles were synthesized by a bio-reduction green method
through the mixing of silver nitrate and the aqueous extract of wormwood
(Artemisia absinthium) at five different ratios. The acquired
nanoparticles (A. absinthium-Ag NPs) were characterized and their
antifungal activity was tested against three species of potentially
pathogenic yeasts of the Candida genus. The nanoparticles were
polydisperse, with a size range between 2-80 nm. An organic matrix
surrounding the nanoparticles was observed by transmission electron
microscopy. Raman spectra of nanoparticles presented bands associated
with functional groups of compounds such as artemisinin and
alpha-bisabolol, present in the organic matrix. ICP-OES results yielded
a decreasing trend in the total silver ions concentration as the silver
nitrate diminished. The antifungal tests showed that four of the five
batches of A. absinthium-AgNPs presented antifungal effects. MIC and MFC
values were lower than the usually reported for Ag nanoparticles
synthesized with other reducing and stabilizing agents. Thus, the
bioactive compounds of wormwood synergistically enhanced the antifungal
activity of the silver nanoparticles. These results suggest the
potential of A. absinthium-AgNPs against some pathogenic yeasts of the
Candida genus.
Filiaciones:
del Pilar Rodriguez-Torres, Maria:
Univ Nacl Autonoma Mexico, Lab Invest Interdisciplinaria, Area Nanoestructuras & Biomat, Escuela Nacl Estudios Super,Unidad Leon, Blvd UNAM 2011, Guanajuato 37684, Mexico
Susana Acosta-Torres, Laura:
Univ Nacl Autonoma Mexico, Lab Invest Interdisciplinaria, Area Nanoestructuras & Biomat, Escuela Nacl Estudios Super,Unidad Leon, Blvd UNAM 2011, Guanajuato 37684, Mexico
Armando Diaz-Torres, Luis:
Ctr Invest Opt AC, AP 1-948, Guanajuato 37150, Mexico
Hernandez Padron, Genoveva:
Univ Nacl Autonoma Mexico, Ctr Fis Aplicada & Tecnol Avanzada, Blvd Juriquilla 3001, Queretaro 76230, Queretaro, Mexico
Garcia-Contreras, Rene:
Univ Nacl Autonoma Mexico, Lab Invest Interdisciplinaria, Area Nanoestructuras & Biomat, Escuela Nacl Estudios Super,Unidad Leon, Blvd UNAM 2011, Guanajuato 37684, Mexico
Millan-Chiu, Blanca E.:
Univ Nacl Autonoma Mexico, Ctr Fis Aplicada & Tecnol Avanzada, CONACyT, Blvd Juriquilla 3001, Queretaro 76230, Queretaro, Mexico
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