Genetic and cytotoxic damage induced by pesticides in greenhouse workers in Atlixco, Puebla, Mexico
Por:
Daniel Ortega-Martinez, Luis, Perez-Armendariz, Beatriz, Waliszewski, Stefan, Gomez-Arroyo, Sandra, del Rocio Banos-Lara, Ma, Teran-Cervantes, Marisol, Castro-Carranza, Gabriel, del Carmen Martinez-Valenzuela, Maria
Publicada:
1 ene 2019
Resumen:
The monitoring of genotoxic damage in laborers of greenhouses allows the
identification of deterioration of DNA originated by working conditions.
It has been suggested the use of different biological markers to
evaluate the risk of occupational exposure and DNA damage, one of them
is the micronucleus (MN) assay. The objective of the study was to
determine the frequency of MN and nuclear abnormalities in the buccal
mucosa epithelium of 40 workers of high and low technology greenhouses,
who are exposed to diferent kinds of pesticides, and of 40 people not
exposed to these compounds, both populations from Atlixco City, in
Puebla, Mexico. The samples taken were air-dried and fixed with
methanol-acetic acid. Later they were stained by the Feulgen reaction.
Nuclear abnormalities were assessed by counting one thousand
differentiated basal cells per participant. Comparison of biomarkers
frequencies indicates higher values of nuclear abnormalities
statistically significant in laborers of greenhouses, whose frequency
index ratios were: 63.0 binucleate cells, 14.2 cells with condensed
chromatin, 8.0 cariolitic cells, 3.8 cells pycnotic, 2.4 cells with
nuclear outbreaks, and 2.3 cells with micronuclei. The highest frequency
of nuclear abnormalities was determined in laborers working in low-tech
greenhouses compared to high-tech greenhouses. The male population of
low-tech greenhouses showed higher frequencies of pycnotic cells,
micronuclei, and cells with nuclear outbreaks.
Filiaciones:
Daniel Ortega-Martinez, Luis:
Univ Popular Autonoma Estado Puebla, Dept Ciencias Biol, 11 Poniente 2316, Puebla 72410, Puebla, Mexico
Perez-Armendariz, Beatriz:
Univ Popular Autonoma Estado Puebla, Dept Ciencias Biol, 11 Poniente 2316, Puebla 72410, Puebla, Mexico
Waliszewski, Stefan:
Univ Veracruzana, Ctr Invest Biomed, Xalapa 91190, Veracruz, Mexico
Gomez-Arroyo, Sandra:
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Circuito Invest Cientif S-N,Ciudad Univ, Coyoacan 04510, Ciudad De Mexic, Mexico
del Rocio Banos-Lara, Ma:
Univ Popular Autonoma Estado Puebla, Ctr Invest Oncol Nueva Esperanza, Venustiano Carranza 504, Puebla 72197, Puebla, Mexico
Teran-Cervantes, Marisol:
Univ Autonoma Occidente, Unidad Invest Ambiente & Salud, Bulevar Macario Gaxiola & Carretera Int, Los Mochis 81223, Sinaloa, Mexico
Castro-Carranza, Gabriel:
Univ Autonoma Occidente, Unidad Invest Ambiente & Salud, Bulevar Macario Gaxiola & Carretera Int, Los Mochis 81223, Sinaloa, Mexico
del Carmen Martinez-Valenzuela, Maria:
Univ Autonoma Occidente, Unidad Invest Ambiente & Salud, Bulevar Macario Gaxiola & Carretera Int, Los Mochis 81223, Sinaloa, Mexico
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