Differential adhesion and fibrinolytic activity of mesenchymal stem cells from human bone marrow, placenta, and Wharton’s jelly cultured in a fibrin hydrogel
Por:
Chaires-Rosas, Casandra P., Ambriz, Xochitl, Montesinos, Juan J., Hernandez-Tellez, Beatriz, Piñón-Zárate G., Herrera-Enriquez, Miguel, Hernandez-Estevez, Erika, Ambrosio, Javier R., Castell-Rodriguez, Andres
Publicada:
1 ene 2019
Resumen:
Mesenchymal stem cells isolated from different tissues should share associated markers and the capability to differentiate to mesodermal lineages. However, their behavior varies in specific microenvironments. Herein, adhesion and fibrinolytic activity of mesenchymal stem cells from placenta, bone marrow, and Wharton’s jelly were evaluated in fibrin hydrogels prepared with nonpurified blood plasma and compared with two-dimensional cultures. Despite the source, mesenchymal stem cells adhered through focal adhesions positive for vinculin and integrin aV in two dimensions, while focal adhesions could not be detected in fibrin hydrogels. Moreover, some cells could not spread and stay rounded. The proportions of elongated and round phenotypes varied, with placenta mesenchymal stem cells having the lowest percentage of elongated cells (~10%). Mesenchymal stem cells degraded fibrin at distinct rates, and placenta mesenchymal stem cells had the strongest fibrinolytic activity, which was achieved principally through the plasminogen–plasmin axis. These findings might have clinical implications in tissue engineering and wound healing therapy. © The Author(s) 2019.
Filiaciones:
Chaires-Rosas, Casandra P.:
Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Cellular & Tissue Biol, Fac Med, Ave Univ 3000, Mexico City 04510, DF, Mexico
Ambriz, Xochitl:
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Montesinos, Juan J.:
Oncology Research Unit, Oncology Hospital, National Medical Center, Mexican Social Security Institute, Mexico City, Mexico
Mexican Social Secur Inst, Oncol Hosp, Oncol Res Unit, Natl Med Ctr, Mexico City, DF, Mexico
Hernandez-Tellez, Beatriz:
Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Cellular & Tissue Biol, Fac Med, Ave Univ 3000, Mexico City 04510, DF, Mexico
Piñón-Zárate G.:
Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Herrera-Enriquez, Miguel:
Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Cellular & Tissue Biol, Fac Med, Ave Univ 3000, Mexico City 04510, DF, Mexico
Hernandez-Estevez, Erika:
Oncology Research Unit, Oncology Hospital, National Medical Center, Mexican Social Security Institute, Mexico City, Mexico
Mexican Social Secur Inst, Oncol Hosp, Oncol Res Unit, Natl Med Ctr, Mexico City, DF, Mexico
Ambrosio, Javier R.:
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Castell-Rodriguez, Andres:
Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
Univ Nacl Autonoma Mexico, Dept Cellular & Tissue Biol, Fac Med, Ave Univ 3000, Mexico City 04510, DF, Mexico
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