Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels modulates cell migration and wound healing
Por:
Ernesto Bastian-Eugenio, Carlos, Bohorquez-Hernandez, Arlette, Pacheco, Jonathan, Sampieri, Alicia, Asanov, Alexander, Pablo Ocelotl-Oviedo, Jose, Guerrero, Adan, Darszon, Alberto, Vaca, Luis
Publicada:
4 mar 2019
Resumen:
Store-operated calcium entry (SOCE) is an essential calcium influx
mechanism in animal cells. One of the most important auto regulatory
control systems involves calcium-dependent inactivation (CDI) of the
Orai channel, which prevents excessive calcium influx. In the present
study we analyze the role of two channels in the induction of CDI on
Orai1. Here we show that calcium entering through freely diffusing TRPV1
channels induce strong CDI on Orai1 while calcium entering through
P2X(4) channel does not. TRPV1 can induce CDI on Orai1 because both
channels were found in close proximity in the cell membrane. This was
not observed with P2X(4) channels. To our knowledge, this is the first
study demonstrating that calcium arising from different channels may
contribute to the modulation of Orai1 through CDI in freely diffusing
single channels of living cells. Our results highlight the role of
TRPV1mediated CDI on Orai1 in cell migration and wound healing.
Filiaciones:
Ernesto Bastian-Eugenio, Carlos:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Bohorquez-Hernandez, Arlette:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Pacheco, Jonathan:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Sampieri, Alicia:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Asanov, Alexander:
TIRF Labs Inc, 106 Grendon Pl, Cary, NC 27519 USA
Pablo Ocelotl-Oviedo, Jose:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City 62250, DF, Mexico
Guerrero, Adan:
Univ Nacl Autonoma Mexico, Lab Nacl Microscopia Avanzada, Mexico City 62250, DF, Mexico
Darszon, Alberto:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Mexico City 62250, DF, Mexico
Vaca, Luis:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
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