Synthetic cementum protein 1-derived peptide regulates mineralization in vitro and promotes bone regeneration in vivo


Por: Correa, Rodrigo, Arenas, Jesus, Montoya, Gonzalo, Hoz, Lia, Lopez, Sonia, Salgado, Fabiola, Arroyo, Rita, Salmeron, Nahum, Romo, Enrique, Zeichner-David, Margarita, Arzate, Higinio

Publicada: 1 ene 2019
Resumen:
The use of recombinant proteins has revolutionized the development of biologic pharmaceuticals; however, they are not free of complications. Some have very high molecular weight, some demonstrate in vivo instability, and the high cost of producing them remains a major problem. On the other hand, it has been shown that peptides derived from active domains keep their biologic activity and can trigger events, such as osteogenesis and bone regeneration. Small peptides are advantageous because of their ease of synthesis and handling and their low immunogenic activity. The purpose of this study was to investigate the functions of a synthetic peptide, cementum protein 1-peptide1 (CEMP-1-p1), both in vitro and in vivo. Our results show that CEMP-1-p1 significantly enhanced the proliferation and differentiation of human periodontal ligament cells toward a mineralizing-like phenotype, as evidenced by increasing alkaline phosphatase (ALP)-specific activity and osterix, runt-related transcription factor (RUNX)-2, integrin binding sialoprotein, bone morphogenetic protein-2, osteocalcin, and cementum protein (CEMP)-1 expression at mRNA and protein levels. In vivo assays performed through standardized critical-size calvarial defects in rats treated with CEMP-1-p1 resulted in newly formed bone after 30 and 60 d. These data demonstrate that CEMP-1-p1 is an effective bioactive peptide for bone tissue regeneration. The application of this bioactive peptide may lead to implementing new strategies for the regeneration of bone and other mineralized tissues.Correa, R., Arenas, J., Montoya, G., Hoz, L., Lopez, S., Salgado, F., Arroyo, R., Salmeron, N., Romo, E., Zeichner-David, M., Arzate, H. Synthetic cementum protein 1-derived peptide regulates mineralization in vitro and promotes bone regeneration in vivo.

Filiaciones:
Correa, Rodrigo:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Arenas, Jesus:
 Instituto de Fisica, Universidad Nacional Autonoma de M Exico (UNAM), Mexico City, Mexico

 Univ Nacl Autonoma Mexico, Inst Fis, Mexico City, DF, Mexico

Montoya, Gonzalo:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Hoz, Lia:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Lopez, Sonia:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Salgado, Fabiola:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Arroyo, Rita:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Salmeron, Nahum:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Romo, Enrique:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico

Zeichner-David, Margarita:
 Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, United States

 Univ Southern Calif, Ostrow Sch Dent, Los Angeles, CA USA

Arzate, Higinio:
 Laboratorio de Biolo?ia Periodontal, Facultad de Odontolo?ia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 3000, C.P., Mexico City, 04510, Mexico

 Univ Nacl Autonoma Mexico, Lab Biol Periodontal, Fac Odontol, Ave Univ 3000, Mexico City 04510, DF, Mexico
ISSN: 08926638
Editorial
FEDERATION AMER SOC EXP BIOL, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 33 Número: 1
Páginas: 1167-1178
WOS Id: 000457401500092
ID de PubMed: 30113883