MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems
Por:
Raya-Gonzalez, Javier, Oropeza-Aburto, Araceli, Lopez-Bucio, Jesus S., Guevara-Garcia, Angel A., de Veylder, Lieven, Lopez-Bucio, Jose, Herrera-Estrella, Luis
Publicada:
1 dic 2018
Resumen:
The Mediator (MED) complex plays a key role in the recruitment and
assembly of the transcription machinery for the control of gene
expression. Here, we report on the role of MEDIATOR18 (MED18) subunit in
root development, auxin signaling and meristem cell viability in
Arabidopsis thaliana seedlings. Loss-of-function mutations in MED18
reduce primary root growth, but increase lateral root formation and root
hair development. This phenotype correlates with alterations in cell
division and elongation likely caused by an increased auxin response and
transport at the root tip, as evidenced by DR5:GFP, pPIN1::PIN1-GFP,
pPIN2::PIN2-GFP and pPIN3::PIN3-GFP auxin-related gene expression.
Noteworthy, med18 seedlings manifest cell death in the root meristem,
which exacerbates with age and/or exposition to DNA-damaging agents, and
display high expression of the cell regeneration factor ERF115. Cell
death in the root tip was reduced in med18 seedlings grown in darkness,
but remained when only the shoot was exposed to light, suggesting that
MED18 acts to protect root meristem cells from local cell death, and/or
in response to root-acting signal(s) emitted by the shoot in response to
light stimuli. These data point to MED18 as an important component for
auxin-regulated root development, cell death and cell regeneration in
root meristems.
Filiaciones:
Raya-Gonzalez, Javier:
Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico
Univ Michoacana, Inst Invest Quim Biol, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico
Oropeza-Aburto, Araceli:
Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico
Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico
Lopez-Bucio, Jesus S.:
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
CONACYT, Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
Univ Michoacana, Inst Invest Quim Biol, CONACYT, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico
Guevara-Garcia, Angel A.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
Univ Nacl Autonoma Mexico, Inst Biotechnol, Apartado Postal 510-3, Cuernavaca 62250, Morelos, Mexico
de Veylder, Lieven:
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, 9052, Belgium
Center for Plant Systems Biology, VIB, Gent, 9052, Belgium
Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium
Lopez-Bucio, Jose:
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
CONACYT, Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
Univ Michoacana, Inst Invest Quim Biol, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico
Herrera-Estrella, Luis:
Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico
Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico
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