MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems


Por: Raya-Gonzalez, Javier, Oropeza-Aburto, Araceli, Lopez-Bucio, Jesus S., Guevara-Garcia, Angel A., de Veylder, Lieven, Lopez-Bucio, Jose, Herrera-Estrella, Luis

Publicada: 1 dic 2018
Resumen:
The Mediator (MED) complex plays a key role in the recruitment and assembly of the transcription machinery for the control of gene expression. Here, we report on the role of MEDIATOR18 (MED18) subunit in root development, auxin signaling and meristem cell viability in Arabidopsis thaliana seedlings. Loss-of-function mutations in MED18 reduce primary root growth, but increase lateral root formation and root hair development. This phenotype correlates with alterations in cell division and elongation likely caused by an increased auxin response and transport at the root tip, as evidenced by DR5:GFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP and pPIN3::PIN3-GFP auxin-related gene expression. Noteworthy, med18 seedlings manifest cell death in the root meristem, which exacerbates with age and/or exposition to DNA-damaging agents, and display high expression of the cell regeneration factor ERF115. Cell death in the root tip was reduced in med18 seedlings grown in darkness, but remained when only the shoot was exposed to light, suggesting that MED18 acts to protect root meristem cells from local cell death, and/or in response to root-acting signal(s) emitted by the shoot in response to light stimuli. These data point to MED18 as an important component for auxin-regulated root development, cell death and cell regeneration in root meristems.

Filiaciones:
Raya-Gonzalez, Javier:
 Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico

 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico

 Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico

 Univ Michoacana, Inst Invest Quim Biol, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico

Oropeza-Aburto, Araceli:
 Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico

 Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico

Lopez-Bucio, Jesus S.:
 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico

 CONACYT, Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico

 Univ Michoacana, Inst Invest Quim Biol, CONACYT, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico

Guevara-Garcia, Angel A.:
 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico

 Univ Nacl Autonoma Mexico, Inst Biotechnol, Apartado Postal 510-3, Cuernavaca 62250, Morelos, Mexico

de Veylder, Lieven:
 Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, 9052, Belgium

 Center for Plant Systems Biology, VIB, Gent, 9052, Belgium

 Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

 VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium

Lopez-Bucio, Jose:
 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico

 CONACYT, Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico

 Univ Michoacana, Inst Invest Quim Biol, Edificio B3,Ciudad Univ, Morelia 58030, Michoacan, Mexico

Herrera-Estrella, Luis:
 Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Campus Irapuato, Guanajuato, Mexico

 Ctr Invest Estudios Avanzados IPN, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Campus Irapuato, Guanajuato, Mexico
ISSN: 09607412
Editorial
Blackwell Publishing Ltd, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA, Estados Unidos America
Tipo de documento: Article
Volumen: 96 Número: 5
Páginas: 895-909
WOS Id: 000451173000002
ID de PubMed: 30270572

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