Centromere-associated protein E expresses a novel mRNA isoform in acute lymphoblastic leukemia
Por:
Jimenez-Avila, Cindy E., Villegas-Ruiz, Vanessa, Zapata-Tarres, Marta, Rubio-Portillo, Alejandra E., Perez Lopez, I, Eleazar, Zenteno, Juan C., Juarez-Mendez, Sergio
Publicada:
1 ene 2018
Resumen:
The alternative splicing plays an important role to generate protein
diversity. Recent studies have shown alterations in alternative
splicing, resulting in loss, gain or changes of functions in the
resulting protein. Specific products of alternative splicing are known
to contribute in cancer-related mechanisms, such as angiogenesis,
migration, adhesion and cell proliferation, among others. We using high
density microarrays reported a CENP-E as a one of significant transcript
expressed and potentially is alternatively spliced in cancer. We focus
in validate alternative splicing of CENP-E transcript using RT-PCR and
sequencing in different cancer cell lines. We performed RT-PCR using
specific primers designed to delimit the non-reported alternative
splicing in CENP-E transcript. Our results showed the co-expression of
the variant one and two of CENP-E in all cell lines evaluated. We
detected more expression of variant one than two. Moreover, we identify
an alternative 5'splice site of CENP-E in the exon 38 and was observed
in RoVa cell line. Additionally, we characterized alternative skipping
from exon 20 (NAT-CENP-E), these alternative splicing was observed in
all cell lines evaluated except RoVa. Finally, we corroborate
alternative mRNA splicing in leukemia patients using quantitative
RT-PCR, in 71.8% of the patients NAT-CENP-E is downregulated and 28.2%
is overexpressed.
Filiaciones:
Jimenez-Avila, Cindy E.:
Perez Lopez, Eleazar, I , Natl Inst Pediat, Res Dept, Expt Oncol Lab, Mexico City, DF, Mexico
Villegas-Ruiz, Vanessa:
Perez Lopez, Eleazar, I , Natl Inst Pediat, Res Dept, Expt Oncol Lab, Mexico City, DF, Mexico
Inst Ophthalmol Conde de Valenciana, Res Unit, Genet Dept, Mexico City, DF, Mexico
Zapata-Tarres, Marta:
Natl Inst Pediat, Oncol Dept, Mexico City, DF, Mexico
Rubio-Portillo, Alejandra E.:
Perez Lopez, Eleazar, I , Natl Inst Pediat, Res Dept, Expt Oncol Lab, Mexico City, DF, Mexico
Zenteno, Juan C.:
Inst Ophthalmol Conde de Valenciana, Res Unit, Genet Dept, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Biochem Dept, Mexico City, DF, Mexico
Juarez-Mendez, Sergio:
Perez Lopez, Eleazar, I , Natl Inst Pediat, Res Dept, Expt Oncol Lab, Mexico City, DF, Mexico
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