Lipid droplets in clusters negatively affect Bos indicus embryos during cryopreservation


Por: Patricia Lopez-Damian, Eva, Alfredo Jimenez-Medina, Jose, Angel Lammoglia, Miguel, Arturo Pimentel, Jaime, Teresa Agredano-Moreno, Lourdes, Wood, Chris, Salvador Galina, Carlos, Fiordelisio, Tatiana

Publicada: 1 oct 2018
Resumen:
Embryo transfer using cryopreserved B.indicus embryos results in low pregnancy rates. The low viability of B.indicus embryos is likely a result of their low freezability relative to embryos from other species, notably B.taurus. Freezability is probably related to the quantity of lipid droplets in the embryonic cells. However, the mechanism underlying this association is unknown. Using multiphoton and transmission electron microscopy, the proportion, volume, morphology and composition of lipid droplets in fresh and cryopreserved B.indicus and B.taurus embryos were studied. B.indicus embryos have more droplets than B.taurus and a greater percentage of lipid droplets in clusters (34% vs. 24%; p<0.05). Also, B.indicus individual droplets are of greater volume than those of B.taurus (47m(3) vs. 34m(3); p<0.05). After freezing, the percentage of clusters decreased in B.indicus (11% vs. 5%; p<0.05), while in B.taurus they increased (2% vs. 7%; p<0.05). Freezing influenced the lipid droplet morphology, increasing the number of cracked droplets in B.indicus embryos (33% fresh vs. 62% frozen; p<0.05), but not in B.taurus embryos (35% fresh vs. 34% frozen; ns). Regarding composition, saturated lipid droplets prevail in B.indicus embryos, contrary to B.taurus embryos. We observed differences in lipid droplet organization, proportion, volume, morphology and composition between B.indicus and B.taurus embryos which are altered in opposite directions following freezing.

Filiaciones:
Patricia Lopez-Damian, Eva:
 Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico

Alfredo Jimenez-Medina, Jose:
 Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico

Angel Lammoglia, Miguel:
 Univ Veracruzana, Fac Ciencias Biol & Agr, Tuxpan, Mexico

Arturo Pimentel, Jaime:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Cuernavaca, Morelos, Mexico

Teresa Agredano-Moreno, Lourdes:
 Univ Nacl Autonoma Mexico, Dept Biol Celular, Fac Ciencias, Lab Microscopia Elect, Edificio Tlahuizcalpan, Mexico City, DF, Mexico

 Univ Nacl Autonoma Mexico, Lab Nanobiol Celular, Mexico City, DF, Mexico

Wood, Chris:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Cuernavaca, Morelos, Mexico

Salvador Galina, Carlos:
 Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Reprod Anim, Ciudad Univ, Mexico City, DF, Mexico

Fiordelisio, Tatiana:
 Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico
ISSN: 03402096





ANATOMIA HISTOLOGIA EMBRYOLOGIA
Editorial
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, Estados Unidos America
Tipo de documento: Article
Volumen: 47 Número: 5
Páginas: 435-443
WOS Id: 000444224400007
ID de PubMed: 29978506