Lipid droplets in clusters negatively affect Bos indicus embryos during cryopreservation
Por:
Patricia Lopez-Damian, Eva, Alfredo Jimenez-Medina, Jose, Angel Lammoglia, Miguel, Arturo Pimentel, Jaime, Teresa Agredano-Moreno, Lourdes, Wood, Chris, Salvador Galina, Carlos, Fiordelisio, Tatiana
Publicada:
1 oct 2018
Resumen:
Embryo transfer using cryopreserved B.indicus embryos results in low
pregnancy rates. The low viability of B.indicus embryos is likely a
result of their low freezability relative to embryos from other species,
notably B.taurus. Freezability is probably related to the quantity of
lipid droplets in the embryonic cells. However, the mechanism underlying
this association is unknown. Using multiphoton and transmission electron
microscopy, the proportion, volume, morphology and composition of lipid
droplets in fresh and cryopreserved B.indicus and B.taurus embryos were
studied. B.indicus embryos have more droplets than B.taurus and a
greater percentage of lipid droplets in clusters (34% vs. 24%;
p<0.05). Also, B.indicus individual droplets are of greater volume than
those of B.taurus (47m(3) vs. 34m(3); p<0.05). After freezing, the
percentage of clusters decreased in B.indicus (11% vs. 5%; p<0.05),
while in B.taurus they increased (2% vs. 7%; p<0.05). Freezing
influenced the lipid droplet morphology, increasing the number of
cracked droplets in B.indicus embryos (33% fresh vs. 62% frozen;
p<0.05), but not in B.taurus embryos (35% fresh vs. 34% frozen; ns).
Regarding composition, saturated lipid droplets prevail in B.indicus
embryos, contrary to B.taurus embryos. We observed differences in lipid
droplet organization, proportion, volume, morphology and composition
between B.indicus and B.taurus embryos which are altered in opposite
directions following freezing.
Filiaciones:
Patricia Lopez-Damian, Eva:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico
Alfredo Jimenez-Medina, Jose:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico
Angel Lammoglia, Miguel:
Univ Veracruzana, Fac Ciencias Biol & Agr, Tuxpan, Mexico
Arturo Pimentel, Jaime:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Cuernavaca, Morelos, Mexico
Teresa Agredano-Moreno, Lourdes:
Univ Nacl Autonoma Mexico, Dept Biol Celular, Fac Ciencias, Lab Microscopia Elect, Edificio Tlahuizcalpan, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Nanobiol Celular, Mexico City, DF, Mexico
Wood, Chris:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Lab Nacl Microscopia Avanzada, Cuernavaca, Morelos, Mexico
Salvador Galina, Carlos:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Reprod Anim, Ciudad Univ, Mexico City, DF, Mexico
Fiordelisio, Tatiana:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Ecol & Recursos Nat Biol, Lab Neuroendocrinol Comparada, Ciudad Univ, Mexico City 04510, DF, Mexico
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