Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia
Por:
Tomas-Sanchez C., Blanco-Alvarez V.-M., Martinez-Fong D., Gonzalez-Barrios J.-A., Gonzalez-Vazquez A., Aguilar-Peralta A.-K., Torres-Soto M., Soto-Rodriguez G., Limón I.D., Brambila E., Millán-Pérez-Peña L., Cebada J., Orozco-Barrios C.E., Leon-Chavez B.A.
Publicada:
1 ene 2018
Resumen:
In the cerebral hypoxia-ischemia rat model, the prophylactic administration of zinc can cause either cytotoxicity or preconditioning effect, whereas the therapeutic administration of selenium decreases the ischemic damage. Herein, we aimed to explore whether supplementation of low doses of prophylactic zinc and therapeutic selenium could protect from a transient hypoxic-ischemic event. We administrated zinc (0.2 mg/kg of body weight; ip) daily for 14 days before a 10 min common carotid artery occlusion (CCAO). After CCAO, we administrated sodium selenite (6 µg/kg of body weight; ip) daily for 7 days. In the temporoparietal cerebral cortex, we determined nitrites by the Griess method and lipid peroxidation by the Gerard-Monnier assay. qPCR was used to measure mRNA of nitric oxide synthases, antioxidant enzymes, chemokines, and their receptors. We measured the enzymatic activity of SOD and GPx and protein levels of chemokines and their receptors by ELISA. We evaluated long-term memory using the Morris-Water maze test. Our results showed that prophylactic administration of zinc caused a preconditioning effect, decreasing nitrosative/oxidative stress and increasing GPx and SOD expression and activity, as well as eNOS expression. The therapeutic administration of selenium maintained this preconditioning effect up to the late phase of hypoxia-ischemia. Ccl2, Ccr2, Cxcl12, and Cxcr4 were upregulated, and long-term memory was improved. Pyknotic cells were decreased suggesting prevention of neuronal cell death. Our results show that the prophylactic zinc and therapeutic selenium administration induces effective neuroprotection in the early and late phases after CCAO.
Filiaciones:
Tomas-Sanchez C.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Blanco-Alvarez V.-M.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Martinez-Fong D.:
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, 07000 Mexico City, Mexico
Gonzalez-Barrios J.-A.:
Laboratorio de Medicina Genómica, Hospital Regional 1° de Octubre, ISSSTE, Avenida Instituto Politécnico Nacional No. 1669, Mexico City, 07760, Mexico
Gonzalez-Vazquez A.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Aguilar-Peralta A.-K.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Torres-Soto M.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Soto-Rodriguez G.:
Facultad de Medicina, Benémerita Universidad Autónoma de Puebla, Puebla, PUE 72420, Mexico
Limón I.D.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Brambila E.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Millán-Pérez-Peña L.:
Centro de Química, ICUAP, Benémerita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
Cebada J.:
Facultad de Medicina, Benémerita Universidad Autónoma de Puebla, Puebla, PUE 72420, Mexico
Orozco-Barrios C.E.:
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, 07000 Mexico City, Mexico
Leon-Chavez B.A.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 sur y Av. San Claudio, Puebla, PUE 72570, Mexico
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