Regulation of heme oxygenase and indoleamine by cytokines in cervical cancer cells and natural killer cell cytotoxic activity
Por:
Ortiz-Lazareno P.C., Gómez-Lomcli P., Hernández-Flores G., RodrIguez-Padilla C., Ochoa-Carrillo F.J., BravoCuellar A.
Publicada:
1 ene 2017
Resumen:
To determine the effect of the cytokines interteukin (IL-4), IL-b, Interferon gamma (IFN-y) and tumor necrosis factor (TNF-a) in protein expression of HO-i and IDO in cervical cancer ceLl Lines and the cytotoxicity of NK cells in co-culture with cervix cancer ceLLs pretreated with HO-i and DO inhibitors. Methods: The cervicaL cancer celL Lines HeLa (HPV 18+), SiHa (HPV 16+) and C-33A (HPV-) were treated with 20 ng/mL of IL-4, IL-b, IFN-y and TNF-a for 3 h, 6 h and 12 h, after that protein expression was assessed by flow cytometr The cytotoxic activity of NK ceLLs was performed in reaL time for 4 h. Results: Protein expression of the enzymes were changed at earLy times, increasing the expression of the enzymes in the 3 ceLl Lines. We observed an increase in cytotoxic activity of NK celLs cocuLtured with HeLa ceLLs pretreated with the HO-b inhibitor and in SiHa ceLLs pretreated with the 100 inhibitor. Conclusion: The suppressor enzyme IDO is positiveLy regulated by IL-b 0 in HeLa, SiHa, and NK cytotoxic activity increase in Hela and SiHa celLs pretreated with HO-i and IDO inhibitors respectively, which could be part of tumor escape mechanisms in cervix cancer. © 2017 Sociedad Mexicana de Oncologia.
Filiaciones:
Ortiz-Lazareno P.C.:
Division de Inmunologla, Centro de Investigaciàn Biomédica de Occidente, Guadalajara, Mexico
Gómez-Lomcli P.:
Division de Inmunologla, Centro de Investigaciàn Biomédica de Occidente, Guadalajara, Mexico
Hernández-Flores G.:
Division de Inmunologla, Centro de Investigaciàn Biomédica de Occidente, Guadalajara, Mexico
RodrIguez-Padilla C.:
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo Leon, San Nicolds de los Garza, Mexico
Ochoa-Carrillo F.J.:
Cirujano Ancologo, Instituto Nacional de Cancerologla de Mexico, Ciudad de Mexico, Mexico
BravoCuellar A.:
Division de Inmunologla, Centro de Investigaciàn Biomédica de Occidente, Guadalajara, Mexico
Departamento de Ciencias de la Salud, Centro Universitarlo de Los Altos, Universidad de Guadalajara, TepatitlOn de Morelos, Mexico
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