beta-Cyclodextrin and oleic acid increase levels of dopamine and potentiates oxidative damage in young and adult rat brain
Por:
Calderon Guzman, David, Osnaya Brizuela, Norma, Ortiz Herrera, Maribel, Juarez Olguin, Hugo, Valenzuela Peraza, Armando, Barragan Mejia, Gerardo
Publicada:
25 jul 2018
Resumen:
Background: Cyclodextrins are active pharmaceutical ingredients to treat
neurological diseases by reducing neurotoxicity. The aim of this study
was to test if combined consumption of beta-cyclodextrin (BCD) and Oleic
acid (OA) potentiates brain antioxidant protection.
Methods: Four groups of young Wistar rats, grouped in 6 animals each,
were treated as follows: Group (G) 1, saline solution 0.9% (control);
G2, BCD (0.7 g/kg); G3, OA (15 ml/kg); G4, BCD + OA. The same design was
assayed for groups of adult rats. Treatments were daily administered by
oral means for five consecutive days. On the last day of administration,
brains of the animals were extracted to measure dopamine, 5-HIAA,
glutathione (GSH), ATPase, Lipoperoxidation and H2O2.
Results: Oleic acid and beta-cyclodextrin upgraded the levels of
dopamine, 5-HIAA and lipid peroxidation and downgraded the
concentrations of GSH and H2O2 in cortex, hemispheres (striatum) and
cerebellum/medulla oblongata regions.
Conclusions: The results of the present study suggest that combined use
of oleic acid and beta-cyclodextrin may increase oxidative damage in
brain regions and promote alteration in dopamine and 5-HIAA amines and
hence, constitutes health risks among age of subjects.
Filiaciones:
Calderon Guzman, David:
INP, Lab Neuroquim, Mexico City, DF, Mexico
Osnaya Brizuela, Norma:
INP, Lab Neuroquim, Mexico City, DF, Mexico
Ortiz Herrera, Maribel:
INP, Lab Bacteriol Expt, Mexico City, DF, Mexico
Juarez Olguin, Hugo:
Univ Nacl Autonoma Mexico, INP, Fac Med, Lab Farmacol, Ave Iman 1,3rd Piso Colonia Cuicuilco CP, Mexico City 04530, DF, Mexico
Valenzuela Peraza, Armando:
INP, Lab Neuroquim, Mexico City, DF, Mexico
Barragan Mejia, Gerardo:
INP, Lab Neuroquim, Mexico City, DF, Mexico
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