The Diversity of Chemoprotective Glucosinolates in Moringaceae (Moringa spp.)
Por:
Fahey, Jed W., Olson, Mark E., Stephenson, Katherine K., Wade, Kristina L., Chodur, Gwen M., Odee, David, Nouman, Wasif, Massiah, Michael, Alt, Jesse, Egner, Patricia A., Hubbard, Walter C.
Publicada:
22 may 2018
Categoría:
Multidisciplinary
Resumen:
Glucosinolates (GS) are metabolized to isothiocyanates that may enhance human healthspan by protecting against a variety of chronic diseases. Moringa oleifera, the drumstick tree, produces unique GS but little is known about GS variation within M. oleifera, and even less in the 12 other Moringa species, some of which are very rare. We assess leaf, seed, stem, and leaf gland exudate GS content of 12 of the 13 known Moringa species. We describe 2 previously unidentified GS as major components of 6 species, reporting on the presence of simple alkyl GS in 4 species, which are dominant in M. longituba. We document potent chemoprotective potential in 11 of 12 species, and measure the cytoprotective activity of 6 purified GS in several cell lines. Some of the unique GS rank with the most powerful known inducers of the phase 2 cytoprotective response. Although extracts of most species induced a robust phase 2 cytoprotective response in cultured cells, one was very low (M. longituba), and by far the highest was M. arborea, a very rare and poorly known species. Our results underscore the importance of Moringa as a chemoprotective resource and the need to survey and conserve its interspecific diversity. © 2018 The Author(s).
Filiaciones:
Fahey, Jed W.:
Johns Hopkins Univ, Cullman Chemoprotect Ctr, Baltimore, MD 21218 USA
Johns Hopkins Univ, Sch Med, Dept Med, Div Clin Pharmacol, Baltimore, MD 21205 USA
Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol, Baltimore, MD 21218 USA
Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Int Hlth, Ctr Human Nutr, Baltimore, MD 21218 USA
Olson, Mark E.:
Univ Nacl Autonoma Mexico, Inst Biol, Tercer Circuito Ciudad Univ, Mexico City 04510, DF, Mexico
Int Moringa Germplasm Collect, Ejido De La Reforma Agr, Jalisco, Mexico
Stephenson, Katherine K.:
Johns Hopkins Univ, Cullman Chemoprotect Ctr, Baltimore, MD 21218 USA
Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol, Baltimore, MD 21218 USA
Wade, Kristina L.:
Johns Hopkins Univ, Cullman Chemoprotect Ctr, Baltimore, MD 21218 USA
Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol, Baltimore, MD 21218 USA
Chodur, Gwen M.:
Johns Hopkins Univ, Cullman Chemoprotect Ctr, Baltimore, MD 21218 USA
Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Int Hlth, Ctr Human Nutr, Baltimore, MD 21218 USA
Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA USA
Odee, David:
Kenya Forestry Res Inst, Biotechnol Lab, Nairobi, Kenya
Nouman, Wasif:
Bahauddin Zakariya Univ, Dept Forestry Range & Wildlife Management, Multan, Pakistan
Massiah, Michael:
George Washington Univ, Dept Chem, Columbian Coll Arts & Sci, Washington, DC 20052 USA
Alt, Jesse:
Johns Hopkins Drug Discovery, Baltimore, MD USA
Egner, Patricia A.:
Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth & Engn, Baltimore, MD USA
Hubbard, Walter C.:
Johns Hopkins Univ, Sch Med, Dept Med, Div Clin Pharmacol, Baltimore, MD 21205 USA
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