TFIIH localization is highly dynamic during zygotic genome activation in Drosophila, and its depletion causes catastrophic mitosis
Por:
Cruz-Becerra, Grisel, Valerio-Cabrera, Sarai, Juarez, Mandy, Bucio-Mendez, Alyeri, Zurita, Mario
Publicada:
1 may 2018
Categoría:
Cell biology
Resumen:
In Drosophila, zygotic genome activation occurs in pre-blastoderm
embryos during rapid mitotic divisions. How the transcription machinery
is coordinated to achieve this goal in a very brief time span is still
poorly understood. Transcription factor II H (TFIIH) is fundamental for
transcription initiation by RNA polymerase II (RNAPII). Herein, we show
the in vivo dynamics of TFIIH at the onset of transcription in
Drosophila embryos. TFIIH shows an oscillatory behaviour between the
nucleus and cytoplasm. TFIIH foci are observed from interphase to
metaphase, and colocalize with those for RNAPII phosphorylated at serine
5 (RNAPIIS5P) at prophase, suggesting that transcription occurs during
the first mitotic phases. Furthermore, embryos with defects in subunits
of either the CAK or the core subcomplexes of TFIIH show catastrophic
mitosis. Although, transcriptome analyses show altered expression of
several maternal genes that participate in mitosis, the global level of
RNAPIIS5P in TFIIH mutant embryos is similar to that in the wild type,
therefore, a direct role for TFIIH in mitosis cannot be ruled out. These
results provide important insights regarding the role of a basal
transcription machinery component when the zygotic genome is activated.
Filiaciones:
Cruz-Becerra, Grisel:
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología . Universidad Nacional Autónoma de México, Av Universidad 2001, Cuernavaca Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Av Univ 2001, Cuernavaca 62250, Morelos, Mexico
Valerio-Cabrera, Sarai:
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología . Universidad Nacional Autónoma de México, Av Universidad 2001, Cuernavaca Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Av Univ 2001, Cuernavaca 62250, Morelos, Mexico
Juarez, Mandy:
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología . Universidad Nacional Autónoma de México, Av Universidad 2001, Cuernavaca Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Av Univ 2001, Cuernavaca 62250, Morelos, Mexico
Bucio-Mendez, Alyeri:
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología . Universidad Nacional Autónoma de México, Av Universidad 2001, Cuernavaca Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Av Univ 2001, Cuernavaca 62250, Morelos, Mexico
Zurita, Mario:
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología . Universidad Nacional Autónoma de México, Av Universidad 2001, Cuernavaca Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Av Univ 2001, Cuernavaca 62250, Morelos, Mexico
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