Neurons and satellite glial cells in adult rat lumbar dorsal root ganglia express connexin 36
Por:
Pérez Armendariz E.M., Norcini M., Hernández-Tellez B., Castell-Rodríguez A., Coronel-Cruz C., Alquicira R.G., Sideris A., Recio-Pinto E.
Publicada:
1 ene 2018
Resumen:
Previous studies have shown that following peripheral nerve injury there
was a downregulation of the gap junction protein connexin 36 (Cx36) in
the spinal cord; however, it is not known whether Cx36 protein is
expressed in the dorsal root ganglia (DRGs), nor if its levels are
altered following peripheral nerve injuries. Here we address these
aspects in the adult rat lumbar DRG. Cx36 mRNA was detected using
qRT-PCR, and Cx36 protein was identified in DRG sections using
immunohistochemistry (IHC) and immunofluorescence (IF). Double staining
revealed that Cx36 co-localizes with both anti-beta-III tubulin, a
neuronal marker, and anti-glutamine synthetase, a satellite glial cell
(SGC) marker. In neurons, Cx36 staining was mostly uniform in somata and
fibers of all sizes and its intensity increased at the cell membranes.
This labeling pattern was in contrast with Cx36 IF dots mainly found at
junctional membranes in islet beta cells used as a control tissue.
Co-staining with anti-Cx43 and anti-Cx36 showed that whereas mostly
uniform staining of Cx36 was found throughout neurons and SGCs, Cx43 IF
puncta were localized to SGCs. Cx36 mRNA was expressed in normal lumbar
DRG, and it was significantly down-regulated in L4 DRG of rats that
underwent sciatic nerve injury resulting in persistent hypersensitivity.
Collectively, these findings demonstrated that neurons and SGCs express
Cx36 protein in normal DRG, and suggested that perturbation of Cx36
levels may contribute to chronic neuropathic pain resulting from a
peripheral nerve injury.
Filiaciones:
Pérez Armendariz E.M.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Torre de Investigación 5to piso, Ciudad Universitaria, Colonia Universidad Nacional Autónoma de México, Avenida Universidad 3000, Circuito InteriorCU, Mexico
Norcini M.:
Department of Anesthesiology, NYULMC, 180 Varick Street, Room 677, New York, NY, United States
Hernández-Tellez B.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Torre de Investigación 5to piso, Ciudad Universitaria, Colonia Universidad Nacional Autónoma de México, Avenida Universidad 3000, Circuito InteriorCU, Mexico
Castell-Rodríguez A.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Torre de Investigación 5to piso, Ciudad Universitaria, Colonia Universidad Nacional Autónoma de México, Avenida Universidad 3000, Circuito InteriorCU, Mexico
Coronel-Cruz C.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Torre de Investigación 5to piso, Ciudad Universitaria, Colonia Universidad Nacional Autónoma de México, Avenida Universidad 3000, Circuito InteriorCU, Mexico
Alquicira R.G.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Torre de Investigación 5to piso, Ciudad Universitaria, Colonia Universidad Nacional Autónoma de México, Avenida Universidad 3000, Circuito InteriorCU, Mexico
Sideris A.:
Department of Anesthesiology, NYULMC, 180 Varick Street, Room 677, New York, NY, United States
Recio-Pinto E.:
Department of Anesthesiology, NYULMC, 180 Varick Street, Room 677, New York, NY, United States
Departments of Anesthesiology, Biochemistry & Molecular Pharmacology, NYULMC, 180 Varick Street, Room 677, New York, NY, United States
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