Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: Association with HDL subclasses
Por:
Luna-Luna M., Cruz-Robles D., Ávila-Vanzzini N., Herrera-Alarcón V., Martínez-Reding J., Criales-Vera S., Sandoval-Zárate J., Vargas-Barrón J., Martínez-Sánchez C., Tovar-Palacio A.R., Fragoso J.M., Carreón-Torres E., Vargas-Alarcón G., Pérez-Méndez Ó.
Publicada:
1 ene 2017
Resumen:
Background: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. Methods: To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. Results: EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque Conclusion: OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. © 2017 The Author(s).
Filiaciones:
Luna-Luna M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Cruz-Robles D.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Ávila-Vanzzini N.:
Department of Echocardiography, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
Herrera-Alarcón V.:
Department of Cardiothoracic Surgery, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
Martínez-Reding J.:
Department of Adult Cardiology, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Criales-Vera S.:
Department of Radiology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Sandoval-Zárate J.:
Department of Cardiopulmonary, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Vargas-Barrón J.:
Department of Echocardiography, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Martínez-Sánchez C.:
Department of Emergency, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Tovar-Palacio A.R.:
Department of Physiology of Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Fragoso J.M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Carreón-Torres E.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Vargas-Alarcón G.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Pérez-Méndez Ó.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, México City, D.F., Mexico
Study Group of Atherosclerosis, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
|