Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism
Por:
Saha S., Weber C.A., Nousch M., Adame-Arana O., Hoege C., Hein M.Y., Osborne-Nishimura E., Mahamid J., Jahnel M., Jawerth L., Pozniakovski A., Eckmann C.R., Jülicher F., Hyman A.A.
Publicada:
1 ene 2016
Categoría:
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Resumen:
P granules are non-membrane-bound RNA-protein compartments that are involved in germline development in C. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how polarity proteins regulate phase separation, we combined biochemistry and theoretical modeling. We reconstitute P granule-like droplets in vitro using a single protein PGL-3. By combining in vitro reconstitution with measurements of intracellular concentrations, we show that competition between PGL-3 and MEX-5 for mRNA can regulate the formation of PGL-3 droplets. Using theory, we show that, in a MEX-5 gradient, this mRNA competition mechanism can drive a gradient of P granule assembly with similar spatial and temporal characteristics to P granule assembly in vivo. We conclude that gradients of polarity proteins can position RNP granules during development by using RNA competition to regulate local phase separation. © 2016 Elsevier Inc.
Filiaciones:
Saha S.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Weber C.A.:
Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Nousch M.:
Martin Luther University, Halle (Saale), Germany
Adame-Arana O.:
Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Hoege C.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Hein M.Y.:
Max Planck Institute of Biochemistry, Martinsried, Germany
Osborne-Nishimura E.:
Colorado State University, Fort Collins, CO, United States
Mahamid J.:
Max Planck Institute of Biochemistry, Martinsried, Germany
Jahnel M.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Jawerth L.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Pozniakovski A.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Eckmann C.R.:
Martin Luther University, Halle (Saale), Germany
Jülicher F.:
Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Hyman A.A.:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
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