Fabrication of low-cost micropatterned polydimethyl-siloxane scaffolds to organise cells in a variety of two-dimensioanl biomimetic arrangements for lab-on-chip culture platforms
Por:
Escutia-Guadarrama, Lidia, Vazquez-Victorio, Genaro, Martinez-Pastor, David, Nieto-Rivera, Brenda, Sosa-Garrocho, Marcela, Macias-Silva, Marina, Hautefeuille, Mathieu
Publicada:
30 nov 2017
Resumen:
We present the rapid-prototyping of type I collagen micropatterns on
poly-dimethylsiloxane substrates for the biomimetic confinement of cells
using the combination of a surface oxidation treatment and 3-aminopropyl
triethoxysilane silanisation followed by glutaraldehyde crosslinking.
The aim of surface treatment is to stabilise microcontact printing
transfer of this natural extracellular matrix protein that usually wears
out easily from poly-dimethylsiloxane, which is not suitable for
biomimetic cell culture platforms and lab-on-chip applications. A
low-cost CD-DVD laser was used to etch biomimetic micropatterns into
acrylic sheets that were in turn replicated to poly-dimethylsiloxane
slabs with the desired features. These stamps were finally inked with
type I collagen for microcontact printing transfer on the culture
substrates in a simple manner. Human hepatoma cells (HepG2) and rat
primary hepatocytes, which do not adhere to bare poly-dimethylsiloxane,
were successfully seeded and showed optimal adhesion and survival on
simple protein micropatterns with a hepatic cord geometry in order to
validate our technique. HepG2 cells also proliferated on the stamps.
Soft and stiff poly-dimethylsiloxane layers were also tested to
demonstrate that our cost-effective process is compatible with
biomimetic organ-on-chip technology integrating tunable stiffness with a
potential application to drug testing probes development where such
cells are commonly used.
Filiaciones:
Escutia-Guadarrama, Lidia:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Nacl Soluc Biomimet Diagnost & Terapia, Mexico City, DF, Mexico
Vazquez-Victorio, Genaro:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Nacl Soluc Biomimet Diagnost & Terapia, Mexico City, DF, Mexico
Martinez-Pastor, David:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
Nieto-Rivera, Brenda:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Sosa-Garrocho, Marcela:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
Macias-Silva, Marina:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Nacl Soluc Biomimet Diagnost & Terapia, Mexico City, DF, Mexico
Hautefeuille, Mathieu:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Nacl Soluc Biomimet Diagnost & Terapia, Mexico City, DF, Mexico
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