Antinociceptive Activity of Ent-Dihydrotucumanoic Acid Isolated from Gymnosperma glutinosum Spreng Less


Por: Josabad Alonso-Castro, Angel, Martin Gonzalez-Chavez, Marco, Ramon Zapata-Morales, Juan, Karina Verdinez-Portales, Alexia, Sanchez-Recillas, Amanda, Ortiz-Andrade, Rolffy, Isiordia-Espinoza, Mario, Martinez-Gutierrez, Fidel, Antonio Ramirez-Morales, Marco, Dominguez, Fabiola, Elizabeth Juache-Flores, Maria, Martinez, Roberto

Publicada: 1 nov 2017
Categoría: Drug discovery

Resumen:
The diterpene ent-dihydrotumanoic acid (DTA) was among the compounds isolated from Gymnosperma glutinosum (Spreng) Less (Asteraceae). There are no reports regarding the pharmacological effects of DTA. Cytotoxicity against cancer cells (1-250 mu M), and the antibacterial (50-1400 mu M) activity of DTA were evaluated using the MTT assay, and the minimum inhibitory concentration test, respectively. The antidiarrheal (1-100 mg/kg p.o.) and anti-inflammatory (2 mg/ear) effects of DTA were evaluated using castor oil and 12-O-tetradecanoylphorbol-13-acetate, respectively. The antinociceptive and sedative effects of DTA (1-100 mg/kg p.o.) were evaluated using two models of chemically-induced nociception, and the pentobarbital-induced sleeping time test, respectively. The antinociceptive mechanism of DTA was evaluated using the acetic acid writhing test with inhibitors related to pain processing pathways. The effects of DTA (10-100 mg/kg p.o.) on locomotor activity were evaluated using the rotarod test. DTA lacked cytotoxic activity (IC50>100 mu M) on cancer cells, possessed moderate antibacterial effects against B. subtillis (MIC= 175 mu M), moderate antidiarrheal and anti-inflammatory effects, and minimal vasorelaxant effects. In the formalin test, DTA showed antinociceptive effects in both phases. In the acetic acid test, DTA showed antinociceptive activity (ED50= 50.2 +/- 5.6 mg/kg) with potency similar to that of naproxen (NPX; ED50=33.7 +/- 4.5 mg/kg) an effect blocked by naloxone implicating an opioid mechanism. DTA also exerted antidiarrheal activity and showed no sedative effects or changes in locomotor activity in mice. Drug Dev Res 78 : 340-348, 2017. (c) 2017 Wiley Periodicals, Inc.

Filiaciones:
Josabad Alonso-Castro, Angel:
 Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Farm, Guanajuato 36050, Guanajuato, Mexico

Martin Gonzalez-Chavez, Marco:
 Univ Autonoma San Luis Potosi, Fac Ciencias Quim, San Luis Potosi, San Luis Potosi, Mexico

Ramon Zapata-Morales, Juan:
 Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Farm, Guanajuato 36050, Guanajuato, Mexico

Karina Verdinez-Portales, Alexia:
 Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Farm, Guanajuato 36050, Guanajuato, Mexico

Sanchez-Recillas, Amanda:
 Univ Autonoma Yucatan, Fac Quim, Yucatan, Mexico

Ortiz-Andrade, Rolffy:
 Univ Autonoma Yucatan, Fac Quim, Yucatan, Mexico

Isiordia-Espinoza, Mario:
 Univ Cuauhtemoc Plantel San Luis Potosi, Escuela Odontol, San Luis Potosi, San Luis Potosi, Mexico

Martinez-Gutierrez, Fidel:
 Univ Autonoma San Luis Potosi, Fac Ciencias Quim, San Luis Potosi, San Luis Potosi, Mexico

Antonio Ramirez-Morales, Marco:
 Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Farm, Guanajuato 36050, Guanajuato, Mexico

Dominguez, Fabiola:
 Inst Mexicano Seguro Social, Ctr Invest Biomed Oriente, Puebla, Mexico

Elizabeth Juache-Flores, Maria:
 Univ Autonoma San Luis Potosi, Fac Ciencias Quim, San Luis Potosi, San Luis Potosi, Mexico

Martinez, Roberto:
 Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Organ, Mexico City, DF, Mexico
ISSN: 02724391
Editorial
Wiley-Liss Inc., DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 78 Número: 7
Páginas: 340-348
WOS Id: 000414678100003
ID de PubMed: 28736816