Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes
Por:
Maria Fernandez-Presas, Ana, Padilla-Noriega, Luis, Ingeborg-Becker, Robert, Lilia, Agustin Jimenez, Jose, Solano, Sandra, Delgado, Jose, Tato, Patricia, Luis Molinari, Jose
Publicada:
1 ene 2017
Resumen:
Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes. © 2017, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.
Filiaciones:
Maria Fernandez-Presas, Ana:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Padilla-Noriega, Luis:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Ingeborg-Becker:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Mexico City, DF, Mexico
Robert, Lilia:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Agustin Jimenez, Jose:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Solano, Sandra:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Delgado, Jose:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Mexico City, DF, Mexico
Tato, Patricia:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000, Mexico City 04510, DF, Mexico
Luis Molinari, Jose:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Bioquim & Biol Estruct, Mexico City, DF, Mexico
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