Leishmania mexicana: promastigotes and amastigotes secrete protein phosphatases and this correlates with the production of inflammatory cytokines in macrophages
Por:
Escalona-Montano, A. R., Ortiz-Lozano, D. M., Rojas-Bernabe, A., Wilkins-Rodriguez, A. A., Torres-Guerrero, H., Mondragon-Flores, R., Mondragon-Gonzalez, R., Becker, I., Gutierrez-Kobeh, L., Aguirre-Garcia, M. M.
Publicada:
1 sep 2016
Resumen:
Phosphatase activity of Leishmania spp. has been shown to deregulate the
signalling pathways of the host cell. We here show that Leishmania
mexicana promastigotes and amastigotes secrete proteins with phosphatase
activity to the culture medium, which was higher in the Promastigote
Secretion Medium (PSM) as compared with the Amastigote Secretion Medium
(ASM) and was not due to cell lysis, since parasite viability was not
affected by the secretion process. The biochemical characterization
showed that the phosphatase activity present in PSM was higher in
dephosphorylating the peptide END (pY) INASL as compared with the
peptide RRA (pT)VA. In contrast, the phosphatase activity in ASM showed
little dephosphorylating capacity for both peptides. Inhibition assays
demonstrated that the phosphatase activity of both PSM and ASM was
sensible only to protein tyrosine phosphatases inhibitors. An antibody
against a protein phosphatase 2C (PP2C) of Leishmania major
cross-reacted with a 44.9 kDa molecule in different cellular fractions
of L. mexicana promastigotes and amastigotes, however, in PSM and ASM,
the antibody recognized a protein about 70 kDa. By electron microscopy,
the PP2C was localized in the flagellar pocket of amastigotes. PSM and
ASM induced the production of tumor necrosis factor alpha, IL-1 beta,
IL-12p70 and IL-10 in human macrophages.
Filiaciones:
Escalona-Montano, A. R.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Ortiz-Lozano, D. M.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Rojas-Bernabe, A.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Wilkins-Rodriguez, A. A.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Torres-Guerrero, H.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Mondragon-Flores, R.:
IPN, CINVESTAV, Ctr Invest & Estudios Avanzados, Dept Bioquim, Av Inst Politecn Nacl 2508, Mexico City, DF, Mexico
Mondragon-Gonzalez, R.:
IPN, CINVESTAV, Ctr Invest & Estudios Avanzados, Dept Genet & Biol Mol, Av Inst Politecn Nacl 2508, Mexico City, DF, Mexico
Becker, I.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Gutierrez-Kobeh, L.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
Aguirre-Garcia, M. M.:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Dr Balmis 148,Colonia Doctores, Mexico City 06726, DF, Mexico
|