alpha-Glucosidase Inhibitors from Salvia circinata


Por: Flores-Bocanegra L., González-Andrade M., Bye R., Linares E., Mata R.

Publicada: 1 may 2017
Resumen:
A dried infusion prepared from the aerial parts of Salvia circinata did not provoke acute toxicity in mice (LD50 > 5 g/kg). This infusion showed poor hypoglycemic and antihyperglycemic effects (100-570 mg/kg) when tested in normal and hyperglycemic mice using acute and oral glucose tolerance tests, respectively. However, this infusion possessed antihyperglycemic action in vivo during an oral sucrose tolerance test (31.6-316 mg/kg), suggesting the presence of a-glucosidase inhibitors in S. circinata. Fractionation of a nonpolar extract of the aerial parts of the plant yielded a new biflavone (1) and four new neoderodane diterpenoid glucosides (2-5) along with the known compounds amarisolide (6), pedalitin (7), apigenin-7-O-beta-D-glucoside (8), and the flavone 2-(3,4-dimethoxypheny1)-5,6-dihydroxy7-methoxy-4H-chromen-4-one (9). Compounds 1 and 6-9 were active against mammalian alpha-glucosidases; 6 and 7 were also active against a recombinant alpha-glucosidase from Ruminococcus obeum and reduced significantly the postprandial peak during an oral sucrose tolerance test in healthy mice, consistent with their alpha-glucosidase inhibitory activity. Molecular docking and dynamic studies revealed that compounds 6 and 7 might bind to alpha-glucosidases at the catalytic center of the enzyme.

Filiaciones:
Flores-Bocanegra L.:
 Univ Nacl Autonoma Mexico, Fac Quim, Cuidad De Mexico 04510, DF, Mexico

 Facultad de Química, Universidad Nacional Autónoma de México, Cuidad de México, 04510, Mexico

González-Andrade M.:
 Univ Nacl Autonoma Mexico, Fac Med, Cuidad De Mexico 04510, DF, Mexico

 Facultad de Medicina, Universidad Nacional Autónoma de México, Cuidad de México, 04510, Mexico

Bye R.:
 Univ Nacl Autonoma Mexico, Inst Biol, Cuidad De Mexico 04510, DF, Mexico

 Instituto de Biología, Universidad Nacional Autónoma de México, Cuidad de México, 04510, Mexico

Linares E.:
 Univ Nacl Autonoma Mexico, Inst Biol, Cuidad De Mexico 04510, DF, Mexico

 Instituto de Biología, Universidad Nacional Autónoma de México, Cuidad de México, 04510, Mexico

Mata R.:
 Univ Nacl Autonoma Mexico, Fac Quim, Cuidad De Mexico 04510, DF, Mexico

 Facultad de Química, Universidad Nacional Autónoma de México, Cuidad de México, 04510, Mexico
ISSN: 01633864
Editorial
American Chemical Society, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 80 Número: 5
Páginas: 1584-1593
WOS Id: 000402498300042
ID de PubMed: 28422509