Phytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves
Por:
Bailon-Moscoso, Natalia, Romero-Benavides, Juan C., Sordo, Monserrat, Villacis, Javier, Silva, Ronald, Celi, Luisa, Martinez-Vazquez, Mariano, Ostrosky-Wegman, Patricia
Publicada:
1 ene 2016
Categoría:
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Resumen:
Some species of the Clusia genus have been shown to have important biomedical properties, including the ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we exam-ined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. & Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO), astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the most substantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induced a genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purification of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol, and hesperidin as active cytotoxic compounds in hexane extract, while ß-amyrine was identified as an active cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further studies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cell survival. © 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.
Filiaciones:
Bailon-Moscoso, Natalia:
Univ Nacl Autonoma Mexico, Dept Med Genom & Toxicol Ambiental, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Univ Tecn Particular Loja, Dept Ciencias Salud, Secc Genet Humana Microbiol & Bioquim Clin, Loja, Ecuador
Romero-Benavides, Juan C.:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
Univ Tecn Particular Loja, Dept Quim, Secc Quim Basica & Aplicada, Loja, Ecuador
Sordo, Monserrat:
Univ Nacl Autonoma Mexico, Dept Med Genom & Toxicol Ambiental, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Villacis, Javier:
Univ Tecn Particular Loja, Dept Ciencias Salud, Secc Genet Humana Microbiol & Bioquim Clin, Loja, Ecuador
Silva, Ronald:
Univ Tecn Particular Loja, Dept Quim, Secc Quim Basica & Aplicada, Loja, Ecuador
Celi, Luisa:
Univ Tecn Particular Loja, Dept Ciencias Salud, Secc Genet Humana Microbiol & Bioquim Clin, Loja, Ecuador
Martinez-Vazquez, Mariano:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
Ostrosky-Wegman, Patricia:
Univ Nacl Autonoma Mexico, Dept Med Genom & Toxicol Ambiental, Inst Invest Biomed, Mexico City 04510, DF, Mexico
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