Nitrogen doped carbon dots derived from Sargassum fluitans as fluorophore for DNA detection
Por:
Godavarthi S., Mohan Kumar K., Vázquez Vélez E., Hernandez-Eligio A., Mahendhiran M., Hernandez-Como N., Aleman M., Martinez Gomez L.
Publicada:
1 jul 2017
Resumen:
This work focused on the use of waste seaweed Sargassum fluitans (S.
fluitans) as carbon source precursor to prepare nitrogen doped carbon
dots (NCDs) by hydrothermal method. High resolution transmission
electron microscopic (HR-TEM) studies revealed that the synthesized
water soluble NCDs are in the size range of 2-8 nm and exhibits
excellent fluorescent properties with a quantum yield of 18.2%.
Elemental nitrogen in NCDs was evidenced by X-ray photoelectron
spectroscopy (XPS) and Fourier transformed infrared spectrum (FT-IR).
The phytochemical analysis of S. fiuitans using H-1 NMR and C-13 NMR
revealed the presence of few amino acids which act as nitrogen source in
the preparation of NCDs. Application of NCDs as fluorophore for double
stranded DNA, single stranded DNA and RNA detection was highlighted in
this study. Excellent fluorescent tagging abilities of NCDs with the
biological nucleic acids were evidenced using gel electrophoresis.
Significant increase in fluorescence was observed upon tagging of NCDs
with nucleic acids and this particular phenomenon helps better in
visualizing the nucleic acids. All three nucleic acids i.e. double
stranded DNA, single stranded DNA and RNA showed similar phenomenon upon
tagging with NCDs. Thus synthesized NCDs may be used as an alternate
fluorophore for commercial toxic organic staining agents to visualize
nucleic acids.
Filiaciones:
Godavarthi S.:
Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico
Univ Juarez Autonoma Tabasco, Conacyt Div Acad Ciencias Basicas, Km 1 Carretera Cunduacan,Jalpa de Mendez AP 24, Cunduacan 86690, Tabasco, Mexico
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico
Conacyt - Division Academica de Ciencias Basicas, Universidad Juarez Autonoma de Tabasco, Km 1 Carretera Cunduacan, Jalpa de Mendez A.P 24 C.P 86690, Colonia Esmeralda, Cunduacan, Tabasco, Mexico
Mohan Kumar K.:
Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico
Vázquez Vélez E.:
Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico
Hernandez-Eligio A.:
UNAM, Inst Biotecnol, CONACYT Dept Bioingn Celular & Biocatalisis, 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
CONACYT - Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Mahendhiran M.:
UNAM, Inst Biotecnol, Dept Bioingn Celular & Biocatalisis, 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico
Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Hernandez-Como N.:
Inst Politecn Nacl, Ctr Nanociencias & Micro & Nanotecnol, Ciudad De Mexico, Mexico
Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Mexico
Aleman M.:
Inst Politecn Nacl, Ctr Nanociencias & Micro & Nanotecnol, Ciudad De Mexico, Mexico
Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Mexico
Martinez Gomez L.:
Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico
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