Nitrogen doped carbon dots derived from Sargassum fluitans as fluorophore for DNA detection


Por: Godavarthi S., Mohan Kumar K., Vázquez Vélez E., Hernandez-Eligio A., Mahendhiran M., Hernandez-Como N., Aleman M., Martinez Gomez L.

Publicada: 1 jul 2017
Resumen:
This work focused on the use of waste seaweed Sargassum fluitans (S. fluitans) as carbon source precursor to prepare nitrogen doped carbon dots (NCDs) by hydrothermal method. High resolution transmission electron microscopic (HR-TEM) studies revealed that the synthesized water soluble NCDs are in the size range of 2-8 nm and exhibits excellent fluorescent properties with a quantum yield of 18.2%. Elemental nitrogen in NCDs was evidenced by X-ray photoelectron spectroscopy (XPS) and Fourier transformed infrared spectrum (FT-IR). The phytochemical analysis of S. fiuitans using H-1 NMR and C-13 NMR revealed the presence of few amino acids which act as nitrogen source in the preparation of NCDs. Application of NCDs as fluorophore for double stranded DNA, single stranded DNA and RNA detection was highlighted in this study. Excellent fluorescent tagging abilities of NCDs with the biological nucleic acids were evidenced using gel electrophoresis. Significant increase in fluorescence was observed upon tagging of NCDs with nucleic acids and this particular phenomenon helps better in visualizing the nucleic acids. All three nucleic acids i.e. double stranded DNA, single stranded DNA and RNA showed similar phenomenon upon tagging with NCDs. Thus synthesized NCDs may be used as an alternate fluorophore for commercial toxic organic staining agents to visualize nucleic acids.

Filiaciones:
Godavarthi S.:
 Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico

 Univ Juarez Autonoma Tabasco, Conacyt Div Acad Ciencias Basicas, Km 1 Carretera Cunduacan,Jalpa de Mendez AP 24, Cunduacan 86690, Tabasco, Mexico

 Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico

 Conacyt - Division Academica de Ciencias Basicas, Universidad Juarez Autonoma de Tabasco, Km 1 Carretera Cunduacan, Jalpa de Mendez A.P 24 C.P 86690, Colonia Esmeralda, Cunduacan, Tabasco, Mexico

Mohan Kumar K.:
 Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico

 Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico

Vázquez Vélez E.:
 Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico

 Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico

Hernandez-Eligio A.:
 UNAM, Inst Biotecnol, CONACYT Dept Bioingn Celular & Biocatalisis, 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico

 CONACYT - Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico

 Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico

Mahendhiran M.:
 UNAM, Inst Biotecnol, Dept Bioingn Celular & Biocatalisis, 2001 Col Chamilpa, Cuernavaca 62210, Morelos, Mexico

 Departamento de Bioingenieria Celular y Biocatalisis, Instituto de Biotecnologia UNAM, No. 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico

Hernandez-Como N.:
 Inst Politecn Nacl, Ctr Nanociencias & Micro & Nanotecnol, Ciudad De Mexico, Mexico

 Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Mexico

Aleman M.:
 Inst Politecn Nacl, Ctr Nanociencias & Micro & Nanotecnol, Ciudad De Mexico, Mexico

 Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Mexico

Martinez Gomez L.:
 Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico

 Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, Cuernavaca, MOR 62210, Mexico
ISSN: 10111344
Editorial
ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND, Suiza
Tipo de documento: Article
Volumen: 172 Número:
Páginas: 36-41
WOS Id: 000403983600005
ID de PubMed: 28514712